文献类型：期刊文献

中文题名：鱼油对顺铂损伤HEK293细胞及A549细胞的影响

英文题名：Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells

作者：杜月梅[1,2];刘云帆[1,2];秦菲[1,2];高丽萍[1,2]

第一作者：杜月梅

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]生物活性物质与功能食品北京市重点实验室,北京100191

第一机构：北京联合大学生物化学工程学院

年份：2022

卷号：43

期号：23

起止页码：347-352

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;EI(收录号：20241015702891);北大核心:【北大核心2020】；

基金：北京联合大学研究生资助项目;北京联合大学推进产教融合,提升实践应用能力项目。

语种：中文

中文关键词：鱼油;顺铂;肾毒性;抗氧化;抗肿瘤

外文关键词：fish oil;cisplatin;nephrotoxicity;anti-oxidant;antitumor

摘要：目的:探讨鱼油对顺铂(cis-dichlorodiamineplatinum(Ⅱ),CDDP)抗A549人肺腺癌细胞活性的影响及对CDDP损伤HEK293细胞的保护作用。方法:体外培养A549和HEK293细胞,将细胞分为对照组、CDDP组、鱼油组、鱼油+CDDP组;分别用不同浓度的鱼油对细胞进行预处理,同时建立CDDP组、鱼油+CDDP组CDDP损伤模型,CCK-8法检测细胞活力,试剂盒测定HEK293细胞中丙二醛(MDA)含量,二还原型谷胱甘肽(GSH)含量,超氧化物歧化酶(SOD)的活力等指标。结果:4~16 mg/L浓度的鱼油能够显著抑制CDDP造成的人胚肾HEK293细胞毒性(P<0.05),其中4 mg/L浓度的鱼油保护效果最佳,且此浓度的鱼油能够显著抑制CDDP(20 mg/L)引起的GSH含量降低以及MDA含量升高(P<0.05)。当鱼油浓度≥32 mg/L时显著增强了CDDP对A549细胞的抑制效果(P<0.05)。结论:一定浓度范围内,鱼油对CDDP致人胚肾细胞HEK293损伤具有一定的保护作用。低浓度鱼油对CDDP所致A549细胞的抑制作用没有明显的影响,高浓度的鱼油增强了CDDP的抗癌效果。
Objective:To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum(Ⅱ)(CDDP)against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP.Methods:A549 and HEK293 cells were cultured in vitro,and the cells were divided into control group,CDDP group,fish oil group,fish oil+CDDP group;the cells were pretreated with different concentrations of fish oil,and CDDP injury models of CDDP group and fish oil+CDDP group were established at the same time.Cell viability was detected by CCK-8 method,and the kits were used to measure malondialdehyde(MDA)content,direduced glutathione(GSH)content,and superoxide dismutase(SOD)activity in HEK293 cells.Results:Fish oil at a concentration of 4~16 mg/L could significantly inhibit the cytotoxicity of HEK293 cells caused by CDDP(P<0.05).Fish oil at a concentration of 4 mg/L had the best protective effect,and fish oil at this concentration could significantly inhibit CDDP(20 mg/L)decreased GSH content and increased MDA content(P<0.05).When the fish oil concentration was≥32 mg/L,the inhibitory effect of CDDP on A549 cells was significantly enhanced(P<0.05).Conclusion:Within a certain concentration range,fish oil has a certain protective effect on CDDP-induced damage to HEK293 of human embryonic kidney cells.Low-concentration fish oil has no obvious effect on the inhibitory effect of CDDP-induced A549 cells,and high-concentration fish oil enhances the anti-cancer effect of CDDP.