文献类型：期刊文献

中文题名：利用丙酮/磷酸氢二钾双水相体系分离纯化绿原酸的研究

英文题名：Separation of Chlorogenic Acid with Aqueous Two-phase Systems Containing Acetone and Dipotassium Hydrogen Phsophate

作者：霍清[1];王强[1]

第一作者：霍清

机构：[1]北京联合大学生物化学工程学院生物医药系

第一机构：北京联合大学生物化学工程学院生物医药系

年份：2008

卷号：29

期号：11

起止页码：234-236

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：双水相萃取;绿原酸;丙酮;磷酸氢二钾

外文关键词：aqueous two-phase systems; chlorogenic acid; acetone; dipotassium hydrogen phosphate

摘要：利用双水相分配技术,研究绿原酸在普通有机溶剂/无机盐体系中的分配行为。利用紫外分光光度计进行定性定量测量,绿原酸的标准曲线为C=0.67649+21.2607A(R=0.99824),限性范围为51μg/mlThe partition behavior of chlorogenic acid in aqueous two-phase systems containing organic solvent and inorganic salt was studied. Ultraviolet spectrophotometer was used for the qualitative and quantitative determination of chlorogenic acid. The standard curve equation is C=0.67649＋21.2607A, R^2=0.99824, with the linear range of 51 μg/ml to 55μg/ml. In this study, the aqueous two-phase systems containing acetone and dipotassium hydrogen phosphate was adopted to extract chlorogenic acid. The effects of quantity of acetone and K2HPO4 ·3H2O on the partition coefficient of chlorogenic acid were investigated. It was found that the optimal extraction conditions of chlorogenic acid with the aqueous two-phase systems are as follows： volume of the aqueous two-phase system 10 ml consisting of acetone 8 ml, water 2 ml and dipotassium hydrogen phosphate 0.8 g. The yield of chlorogenic acid is up to 99.94% under the above conditions. This method easy to restoring, dispose and operate.