文献类型：期刊文献

英文题名：Taurine alleviated hepatic steatosis in oleic acid-treated-HepG2 cells and rats fed a high-fat diet

作者：Song, Qi[1,2];Guo, Jun Xia[1];Ma, Yu Xun[1];Ou, Tong[1];Zhang, Jing[1];Li, Hui Zi[3];Mi, Sheng Quan[1];Zhang, Yan Zhen[1];Oda, Hiroaki[2,5];Chen, Wen[1,4]

第一作者：Song, Qi

通讯作者：Chen, W[1];Oda, H[2]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[2]Nagoya Univ, Lab Nutr Biochem, Nagoya 4648601, Japan;[3]PLA Rocket Force Characterist Med Ctr, Dept Nutr, Beijing 100088, Peoples R China;[4]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beituchengxi Rd 197, Beijing 100191, Peoples R China;[5]Nagoya Univ, Dept Appl Biosci, Lab Nutr Biochem, Nagoya 4648601, Japan

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beituchengxi Rd 197, Beijing 100191, Peoples R China;[2]corresponding author), Nagoya Univ, Dept Appl Biosci, Lab Nutr Biochem, Nagoya 4648601, Japan.|[114172]北京联合大学应用文理学院;[11417]北京联合大学;[1141726]北京联合大学生物化学工程学院;

年份：2023

卷号：9

期号：6

外文期刊名：HELIYON

收录：;Scopus(收录号：2-s2.0-85160443939);WOS:【SCI-EXPANDED(收录号:WOS:001084581004410)】；

基金：We gratefully acknowledge the support of Academic Research Projects of Beijing Union University (XP202005) and Qi Song thanks China CSC Scholarship for financial support (202208050007) .

语种：英文

外文关键词：Taurine; AMP -activated protein kinase (AMPK); Triacylglycerol; Hepatic steatosis

摘要：Taurine has been proven in many trials to alleviate the symptoms of metabolic associated fatty liver disease. Here its protective effect for hepatic steatosis and modulation of AMP-activated protein kinase and insulin signaling pathway were investigated. Steatotic HepG2 cell estab-lished with oleic acid (0.05 mmol/L), treated with taurine (5 mmol/L), dorsomorphin (10 & mu;mol/ L) for 24 h. Sprague Dawley rats were divided into regular and high-fat diet (HFD) groups, and their corresponding taurine (70 or 350 mg/kg BW/d) groups, fed for 8 weeks. In steatotic cell, taurine reduced the TG concentration and SREBP-1c, PPAR & gamma;, FAS, ACC, SCD1 protein levels, decreased phosphorylation of mTOR, IRS1 (Ser302), increased phosphorylation of AMPK & alpha;, LKB1, PI3K, Akt, ACC. While dorsomorphin eliminated taurine's TG-lowering effect. In HFD-fed rats, taurine reduced liver TG, serum TG, ALT, AST, IL-1 & beta;, IL-4, TNF-& alpha;. The effects of taurine on the main factors of fatty acid synthesis were mostly consistent with cell experiments, and the reduction of microRNAs (451, 33, 291b) was aligned with the improvement in LKB1 and AMPK expression in HFD rats. Taurine alleviated steatosis-induced inhibition of IRS1-PI3K-Akt pathway, but suppressed its positively regulated downstream factor mTOR. In parallel, taurine reduced steatosis by activating LKB1-AMPK & alpha; pathway via phosphorylation and no-phosphorylation manner, then inhibiting SREBP-1c directly or by suppressing mTOR phosphorylation.