文献类型：期刊文献

英文题名：Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism

作者：Nie, Xiaojing[1];Sun, Xirao[1];Wang, Chengyue[1];Yang, Jingxin[2]

第一作者：Nie, Xiaojing

通讯作者：Wang, CY[1];Yang, JX[2]

机构：[1]Jinzhou Med Univ, Affiliated Hosp 2, Dept Prosthodont, Jinzhou 121000, Peoples R China;[2]Beijing Union Univ, Coll Robot, Beijing 100000, Peoples R China

第一机构：Jinzhou Med Univ, Affiliated Hosp 2, Dept Prosthodont, Jinzhou 121000, Peoples R China

通讯机构：[1]corresponding author), Jinzhou Med Univ, Affiliated Hosp 2, Dept Prosthodont, Jinzhou 121000, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Robot, Beijing 100000, Peoples R China.|[1141739]北京联合大学机器人学院;[11417]北京联合大学;

年份：2020

卷号：7

期号：1

起止页码：53-62

外文期刊名：REGENERATIVE BIOMATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000569529300007)】；

基金：This work was supported by Science and Technology Fund of Liaoning Province (No.20180530071 and No.2019-MS-141).

语种：英文

外文关键词：magnesium ion; integrin; Type I collagen; FAK; ERK

摘要：Type I collagen (Col I) is a main component of extracellular matrix (ECM). Its safety, biocompatibility, hydrophilicity and pyrogen immunogenicity make it suitable for tissues engineering applications. Mg2+ also control a myriad of cellular processes, including the bone development by enhancing the attachment and differentiation of osteoblasts and accelerating mineralization to enhance bone healing. In our studies, Mg2+ bind collagen to promote the proliferation and differentiation of osteoblasts through the expression of integrins and downstream signaling pathways. In order to clarify the biological behavior effect of 10mM Mg2+/Col I coating, we performed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase (ALP), 4'6-diamidino-2-phenylindole (DAPI), Alizarin red staining and Rhodamine B-isothiocyanate (RITC)-labeled phalloidin experiments and found that 10mM Mg2+ group, Col I-coating group, 10mM Mg2+/Col I-coating group, respectively, promoted the proliferation and differentiation of osteoblasts, especially 10mM Mg2+/Col I-coating group. We detected the mRNA expression of osteogenic-related genes (Runx2, ALP and OCN, OPN and BMP-2) and the protein expression of signaling pathway (integrin alpha 2, integrin beta 1, FAK and ERK1/2), these results indicated that 10mM Mg2+/Col I coating play an critical role in up-regulating the MC3T3-E1 cells activity. The potential mechanisms of this specific performance may be through activating via integrin alpha 2 beta 1-FAK-ERK1/2 protein-coupled receptor pathway.