文献类型：期刊文献

英文题名：Co-production of 1,3-Propanediol and 2,3-Butanediol from Waste Lard by Co-cultivation of Pseudomonas alcaligenes and Klebsiella pneumoniae

作者：Li, Ying[1];Zhu, Siyu[1];Ge, Xizhen[1]

第一作者：李映

通讯作者：Ge, XZ[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：76

期号：4

起止页码：415-424

外文期刊名：CURRENT MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85061434077);WOS:【SCI-EXPANDED(收录号:WOS:000462213700004)】；

基金：This work was financially supported by Joint Funding of Beijing Municipal Natural Science Foundation-Beijing Municipal Education Commission, National Key R&D Program of China [2017YFD0201105] and Premium Funding Project for Academic Human Resources Development in Beijing Union University [BPHR2017DZ07].

语种：英文

摘要：The platform chemicals 1,3-propanediol (1,3-PD) and 2,3-butanediol (2,3-BD) are important raw materials for polyesters and biofuels. However, the biosynthesis of the compounds relies on massive consumption of glucose or glycerol, leading to the uneconomical production in industrial scale. In this work, we developed a new method for co-production of 1,3-PD and 2,3-BD from waste lard to reduce the cost in carbon source supply. A waste lard utilizing Pseudomonas alcaligenes PA-3 and a 1,3-PD producing Klebsiella pneumoniae AA405 were co-cultivated by using waste lard as the sole carbon source. In a shake flask, 1.05g/L 1,3-PD and 0.35g/L 2,3-BD were produced from waste lard within 24h. The addition of nitrogen source significantly increased the relative ratio of K. pneumoniae AA405 in the medium, which further favored to the higher titers of the two products. In bioreactor, the co-cultivation system produced 5.98g/L 1,3-PD and 4.29g/L 2,3-BD from 100g/L waste lard within 72h, and the conversion rate of 1,3-PD and 2,3-BD from waste lard were 62.95% and 0.75%, respectively. In all, this is the first work on 1,3-PD and 2,3-BD production from waste triglyceride, which will favor the utilization of low-cost carbon source in industrial production of chemicals.