文献类型：期刊文献

英文题名：The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

作者：Tong, Tong[1,2];Niu, Xiaohui[1,2];Li, Qian[1,2];Ling, Yuxi[1,2];Li, Zuming[1,2];Liu, Jia[3];Zhang, Michael[4];Bai, Zhihui[5];Xia, Ran[4];Wu, Zhichao[4];Liu, Xiu[6]

通讯作者：Li, ZM[1];Li, ZM[2]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Coll Biochem Engn, Beijing, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Beijing, Peoples R China;[3]Internal Trade Food Sci & Technol Beijing Co Ltd, Beijing, Peoples R China;[4]Univ Manitoba, Dept Phys & Astron, Winnipeg, MB, Canada;[5]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China;[6]China Natl Res Inst Food & Fermentat Ind Co Ltd, Beijing, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Coll Biochem Engn, Beijing, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Beijing, Peoples R China.|[11417146]北京联合大学生物化学工程学院食品科学系;[11417]北京联合大学;[1141726]生物化学工程学院;[114172]北京联合大学应用文理学院;

年份：2021

卷号：70

期号：2

起止页码：235-243

外文期刊名：POLISH JOURNAL OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000664459700008)】；

基金：This paper was supported by Beijing Natural Science Foundation [grant number 6173033], Beijing Union University Foundation [grant number 12213611605-001], Academic Research Projects ofBeijing Union University [grant number ZK70202003], and Internal Trade Food Science and Technology (Beijing) Co., Ltd Cooperation Projects [grant number 202116].

语种：英文

外文关键词：Lactobacillus plantarum; composition; gut microbiota; 16S rRNA amplicon sequencing; BALB/c mice

摘要：Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of L. plantarum BW2013 (a low-dose group of 10(8) CFU/ml, a medium-dose group of 10(9) CFU/ml, and a high-dose group of 10(10) CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in beta diversity between the treatment groups. Compared to the control group, the abundance of Bacteroidetes increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of Alloprevotella increased in the low-dose group compared to the control group. The increased abundance of Ruminococcaceae and decreased abundance of Candidatus_Saccharimonas was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and Alistipes and Candidatus_Saccharimonas decreased in the high-dose group. These results indicated that L. plantarum BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.