文献类型：期刊文献

中文题名：艾绒的燃烧特征及其对骨关节炎大鼠的生物学效应和作用机制研究

英文题名：Thermodynamic characteristics of moxa floss and its biological effects and therapeutic mechanism on osteoarthritis rats

作者：张元[1,2];张弛[1];刘晓宇[1];黄显章[4];张伟萌[1];刘大会[5];郭兰萍[2];黄璐琦[2];康利平[2];李洪梅[3]

第一作者：张元

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700;[3]中国中医科学院中药研究所,北京100700;[4]南阳理工学院河南省张仲景方药与免疫调节重点实验室,河南南阳473004;[5]湖北中医药大学药学院,湖北武汉430065

第一机构：北京联合大学生物化学工程学院

年份：2020

卷号：45

期号：17

起止页码：4071-4080

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：国家重点基础研究发展计划(973)项目(2015CB554506);中央本级重大增减支项目(2060302);黔科合支撑(20172546)。

语种：中文

中文关键词：艾绒;热重分析/示差扫描量热法;艾灸疗法;膝骨关节炎;作用机制

外文关键词：moxa floss;TG/DSC;moxibustion;ostearthritis(OA);therapeutic mechanism

摘要：艾绒是灸法的主体材料,通过燃烧的热效应发挥治疗的作用。为探讨艾绒的燃烧特性,以及探究灸法热疗的疗效作用及作用机制,该文采用TG/DSC的热分析方法研究了蕲春产艾绒的燃烧热特征参数,并通过灸疗膝骨关节炎(ostearthritis,OA)动物模型来研究艾灸的热疗效效果及效应机制。结果表明,艾绒的燃烧过程可分为干燥、燃烧氧化与炭化、燃尽灰分阶段3个过程,艾绒燃烧性质介于草本与木本之间,更趋向木本,具有易燃、缓慢、持久的燃烧特征。艾灸能在一定程度上缓解膝关节滑膜的病理状态,降低OA鼠膝关节肿胀度和血瘀,缩小间质水肿,改善局部炎症。灸疗治疗OA的机制和靶标点可能有上调TRPM3基因激活离子通道,影响钙代谢,减轻OA肿胀程度;下调GAPDH影响膝关节滑膜细胞的糖代谢,介导抗炎作用;下调疼痛相关基因MMP24有助于减轻OA疼痛;上调CTNNB1激活Wnt/β-catenin信号通路,影响OA软骨细胞的分化和再生。该研究首次揭示了艾绒的热解特征并探讨了灸疗热的生物学效应和可能的作用机制,为艾绒的质量评价和灸疗法的作用机制阐述提供了新思路。
Moxa floss is the main material of moxibustion,which plays a therapeutic role through the thermal effect of combustion.In this paper,TG/DSC thermal analysis method was used to study the characteristic parameters of combustion heat of moxa floss produced in Qichun,and the thermal therapeutic effect and mechanism of moxibustion were studied through moxibustion OA animal model.The results show that the combustion process of moxa floss can be divided into three stages:drying,combustion oxidation and carbonization,and ash burnout.The combustion properties of moxa floss are between herbaceous and woody,and tend to be woody,with flammable,slow and lasting combustion characteristics.Moxibustion can relieve the pathological state of knee joint synovium to a certain extent,reduce knee joint swelling and blood stasis in OA rats,reduce interstitial edema,and improve local inflammation.The mechanism and target point of moxibustion treatment for OA may be up-regulating TRPM3 gene to activate ion channels,affecting calcium metabolism and reducing OA swelling degree;down-regulation of GAPDH affects glucose metabolism of knee synovial cells and mediates anti-inflammatory effect.Down-regulation of pain-related gene MMP24 is helpful to relieve OA pain.Up-regulation of CTNNB1 activates Wnt/β-catenin signaling pathway and affects differentiation and regeneration of OA chondrocytes.This study reveals the pyrolysis characteristics of moxa floss for the first time and discusses the biological effect and possible mechanism of moxibustion heat,providing new ideas for the quality evaluation of moxa floss and the mechanism of moxibustion therapy.