文献类型：期刊文献

英文题名：Rapid Identification of 3,6 '-Disinapoyl Sucrose Metabolites in Alzheimer's Disease Model Mice Using UHPLC-Orbitrap Mass Spectrometry

作者：Yuan, Jiaqi[1];Wang, Han[1];Wang, Yunting[1];Wang, Zijian[2];Huo, Qing[1];Dai, Xueling[1];Zhang, Jiayu[3];Sun, Yaxuan[1]

第一作者：Yuan, Jiaqi

通讯作者：Sun, YX[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[2]Beijing Univ Chinese Med, Beijing Res Inst Chinese Med, Beijing 100029, Peoples R China;[3]Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2022

卷号：27

期号：1

外文期刊名：MOLECULES

收录：;Scopus(收录号：2-s2.0-85121672689);WOS:【SCI-EXPANDED(收录号:WOS:000759820100001)】；

基金：This work was supported by the Foundation of China [Grant nos. 11,475,020 and 11,975,048]; National Natural Science, the Graduate Student Scientific Research Innovation Support Project of Beijing Union University (YZ2020K001); Academic Research Projects of Beijing Union University (No. XP202007).

语种：英文

外文关键词：Alzheimer's disease; 3,6 '-disinapoyl sucrose; UHPLC-Orbitrap mass spectrometry; metabolites; metabolic pathway

摘要：Alzheimer's disease (AD) is a degenerative disease of the central nervous system characterized by the progressive impairment of neural activity. Studies have shown that 3,6 & PRIME;-disinapoyl sucrose (DISS) can alleviate the pathological symptoms of AD through the activation of the cAMP/CREB/BDNF signaling pathway. However, the exact biochemical mechanisms of action of DISS are not clear. This study explores metabolism of DISS in an AD mouse model, induced by the microinjection of a lentiviral expression plasmid of the APPswe(695) gene into CA1 of the hippocampus. After gavage administration of DISS (200 mg/kg), the kidneys, livers, brains, plasma, urine, and feces were collected for UHPLC-Orbitrap mass spectrometry analysis. Twenty metabolites, including the prototype drug of DISS, were positively or tentatively identified based on accurate mass measurements, characteristic fragmentation behaviors, and retention times. Thus, the metabolic pathways of DISS in AD mice were preliminarily elucidated through the identification of metabolites, such as ester bond cleavage, demethoxylation, demethylation, and sinapic acid-related products. Furthermore, differences in the in vivo distribution of several metabolites were observed between the model and sham control groups. These findings can provide a valuable reference for the pharmacological mechanisms and biosafety of DISS.