文献类型：期刊文献

中文题名：人源乳杆菌联合菊粉对2型糖尿病小鼠的改善作用

英文题名：Ameliorative Effects of Human-Origin Lactobacillus Combined with Inulin in Type 2 Diabetic Mice

作者：牛晓辉[1];佟童[1];李雨欣[1];张弓[2];刘佳[3];李祖明[1];高丽萍[1];刘秀[4];夏然[3];武志超[3]

第一作者：牛晓辉

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]中加健康工程研究院(合肥)有限公司,安徽合肥230088;[3]国贸食品科技(北京)有限公司,北京102209;[4]中国食品发酵工业研究院有限公司,北京100015

第一机构：北京联合大学生物化学工程学院

年份：2022

卷号：43

期号：21

起止页码：209-217

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;EI(收录号：20225113284783);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：北京市自然科学基金资助项目(6173033);北京联合大学校级科研项目(ZK70202003);“启明星”大学生科技创新创业市级项目(202011417SJ130);中加健康工程研究院(合肥)有限公司合作项目(50304JA1901)。

语种：中文

中文关键词：2型糖尿病;人源乳杆菌;菊粉;磷脂酰肌醇-3-激酶/丝氨酸/苏氨酸激酶通路

外文关键词：type 2 diabetes;human-origin Lactobacillus;inulin;phosphatidylinositol-3-kinase/serine/threonine kinase pathway

摘要：目的:探究人源乳杆菌联合菊粉对2型糖尿病小鼠的改善效果及机制。方法:采用db/db糖尿病模型小鼠,灌胃4株人源乳杆菌混合菌剂(10 mL/(kg m_(b)·d),每株菌的活菌数约1.0×10^(9) CFU/mL),基础饲料添加质量分数5%菊粉,对小鼠进行饮食干预,每周测定小鼠体质量、摄食量和空腹血糖浓度;干预末期进行小鼠口服葡萄糖耐量试验和胰岛素耐受试验;实验结束后测定小鼠空腹血糖浓度、血脂指标、糖化血清蛋白浓度、炎症因子mRNA相对表达水平、磷脂酰肌醇-3-激酶(phosphatidylinositol-3-kinase,PI3K)和丝氨酸/苏氨酸激酶(serine/threonine kinase,AKT)相对表达水平;取小鼠胰腺、肾脏和肝脏采用苏木精-伊红染色并观察组织形态。结果:人源乳杆菌联合菊粉干预可有效调节糖尿病模型小鼠血糖浓度和血脂指标水平,降低胰岛素抵抗和糖化血清蛋白浓度;抑制促炎因子白细胞介素(interleukin,IL)-6和IL-1β的mRNA表达,促进抑炎因子IL-10的mRNA表达;上调PI3K和AKT蛋白的表达;对肝脏、肾脏和胰腺的损伤有明显的改善作用。结论:人源乳杆菌联合菊粉通过有效降低胰岛素抵抗、抑制炎症反应和促进PI3K/AKT信号通路表达改善2型糖尿病小鼠症状。
Objective:To investigate the ameliorative effect and underlying mechanism of human-origin Lactobacillus combined with inulin in type 2 diabetic mice(T2DM).Methods:Obese/diabetic(db/db)mice were consecutively administrated with a cocktail of four strains(each with a viable count of 1.0×10^(9) CFU/mL)of human-origin Lactobacillus at a dose of 10 mL/(kg m_(b)·d),and fed a basal incorporated with 5%inulin for dietary intervention on mice.Body mass,feed intake and fasting blood glucose levels were measured once a week.At the late stage of intervention,oral glucose tolerance and insulin resistance tests were performed.After completion of the experiment,fasting blood glucose,blood lipids,glycated serum protein(GSP),the mRNA expression of inflammatory factors,and phosphatidylinositol-3-kinase(PI3K)and serine/threonine kinase(AKT)protein expression were measured.Liver,kidney and pancreas tissues were collected and stained with hematoxylin-eosin(HE)to observe histomorphology.Results:Human-origin Lactobacillus coordinated with inulin effectively regulated blood glucose and lipid levels,reduced the expression of GSP and improved insulin resistance.The mRNA expression of pro-inflammatory factors interleukin(IL)-6 and IL-1βwere significantly inhibited and the mRNA expression of anti-inflammatory factor IL-10 was promoted.Furthermore,the expression of PI3K and AKT proteins were increased significantly,and pathological damage was significantly improved in liver,kidney and pancrea tissues.Conclusion:Human-origin Lactobacillus coordinated with inulin can alleviate type 2 diabetic symptoms in mice by reducing insulin resistance and inflammatory response,and activating the PI3K/AKT signaling pathway.