文献类型：期刊文献

英文题名：Loss of Serine-Type D-Ala-D-Ala Carboxypeptidase DacA Enhances Prodigiosin Production in Serratia marcescens

作者：Pan, Xuewei[1];Sun, Changhao[1];Tang, Mi[1];Liu, Chao[1];Zhang, Jianing[1];You, Jiajia[1];Osire, Tolbert[1];Sun, Yang[1];Zhao, Youxi[2];Xu, Meijuan[1];Yang, Taowei[1];Rao, Zhiming[1]

第一作者：Pan, Xuewei

通讯作者：Yang, TW[1];Rao, ZM[1]

机构：[1]Jiangnan Univ, Sch Biotechnol, Lab Appl Microorganisms & Metab Engn, Key Lab Ind Biotechnol,Minist Educ, Wuxi, Jiangsu, Peoples R China;[2]Beijing Union Univ, Biochem Engn Coll, Beijing, Peoples R China

第一机构：Jiangnan Univ, Sch Biotechnol, Lab Appl Microorganisms & Metab Engn, Key Lab Ind Biotechnol,Minist Educ, Wuxi, Jiangsu, Peoples R China

通讯机构：[1]corresponding author), Jiangnan Univ, Sch Biotechnol, Lab Appl Microorganisms & Metab Engn, Key Lab Ind Biotechnol,Minist Educ, Wuxi, Jiangsu, Peoples R China.

年份：2019

卷号：7

外文期刊名：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000502955400001)】；

基金：This work was funded by the National Key Research and Development Program of China (2018YFA0900304), National Natural Science Foundation of China (31870066, 21778024, 31770058), National first-class discipline program of Light Industry Technology and Engineering (LITE2018-06), Key research and development program of Ningxia Hui autonomous region (2017BY069), the Program of Introducing Talents of Discipline to Universities (111-2-06), the Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (KLIB-KF201705), and the Innovation project of Jiangsu Province (KYCX17_1419).

语种：英文

外文关键词：D-Ala-Ala carboxypeptidase DacA; prodigiosin synthesis; prodigiosin leakage; feedback inhibition; Serratia marcescens

摘要：Serratia marcescens, a gram-negative bacterium, found in a wide range of ecological niches can produce several high-value products, including prodigiosin, althiomycin, and serratamolide. Among them, prodigiosin has attracted attention due to its immunosuppressive, antimicrobial, and anticancer properties. However, the regulatory mechanisms behind prodigiosin synthesis in Serratia marcescens remains limited. Here, a transposon mutant library was constructed to identify the genes related to prodigiosin synthesis, and BVG90_02415 gene encoding a peptidoglycan synthesizing enzyme D-Ala-D-Ala carboxypeptidase DacA was found to negatively regulates prodigiosin synthesis. Quantitative measurements revealed that disruption of dacA increased prodigiosin production 1.46-fold that of the wild-type strain JNB5-1 in fermentation medium. By comparing differences in cell growth, pigA gene expression level, cell morphology, membrane permeability, and intracellular prodigiosin concentration between wild-type strain JNB5-1 and dacA mutant SK4-72, results revealed that the mechanism for hyper-producing of prodigiosin by the dacA mutant was probably that dacA disruption enhanced prodigiosin leakage, which in turn alleviated feedback inhibition of prodigiosin and increased expression of pig gene cluster. Collectively, this work provides a novel insight into regulatory mechanisms of prodigiosin synthesis and uncovers new roles of DacA protein in regulating cell growth, cell morphology, and membrane permeability in Serratia marcescens. Finally, this study offers a new strategy for improving production of high-value compounds in Serratia marcescens.