文献类型：期刊文献

英文题名：Simultaneous Determination of Promethazine and its Metabolites by Improved Capillary Electrophoresis Coupled with Electrochemiluminescence

作者：Yang, Fuxiu[1];Zhou, Kaowen[1,2];Lu, Yun[3];Yoshida, Hiroyuki[4];Yang, Hongwei[1,2]

第一作者：Yang, Fuxiu

通讯作者：Zhou, KW[1];Yang, HW[1];Zhou, KW[2];Yang, HW[2];Lu, Y[3]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[3]Chiba Univ, Grad Sch Sci & Engn, Dept Mech Engn, Inage Ku, 1-33 Yayoi Cho, Chiba 2638522, Japan;[4]Chiba Ind Technol Res Inst, Inage Ku, 6-13-1 Tendai, Chiba 2630016, Japan

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[3]corresponding author), Chiba Univ, Grad Sch Sci & Engn, Dept Mech Engn, Inage Ku, 1-33 Yayoi Cho, Chiba 2638522, Japan.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：14

期号：9

起止页码：9159-9169

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85072863927);WOS:【SCI-EXPANDED(收录号:WOS:000483307100066)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No. 2152013) and State 863 Program of China (2015AA020200).

语种：英文

外文关键词：Improved capillary electrophoresis; Electrochemiluminescence; Promethazine; Metabolite; Urine sample

摘要：Promethazine sulfoxide (PMZSO), dioxopromethazine (DOPMZ) and demethylpromethazine (DMPMZ) are all the main metabolites of promethazine (PMZ) in vivo. DMPMZ has only one methyl less than PMZ. The structural difference between PMZ and DMPMZ is too small to separate them by conventional capillary electrophoresis (CE). However, the separation efficiency of PMZ and DMPMZ can be improved by adjusting the composition of separation buffer. Combined with electrochemiluminescence (CEL) detection technology, a simultaneous determination method of PMZ, PMZSO, DOPMZ and DMPMZ by improved CE coupled with end-column CEL was established. The parameters about CE separation and CEL detection were investigated in detail. The optimum experimental conditions were detection potential 1.20 V (vs. Ag/AgCl), 40 mmol/L of phosphate buffer solution (pH 6.5) containing 6 mmol/L Ru(bpy)(3)(2+) in CEL detection cell, separation buffer of 21.5% iso-propyl alcohol aqueous solution (v/v) containing 20 mmol/L phosphate (pH 5.0), separation voltage of 14 kV, sample injection time of 7 s and sample injection voltage of 12 kV. The limits of detection (3 sigma) of this method were 7.3x10(-7) mg/mL for PMZ, 8.5x10(-6) mg/mL for PMZSO, 1.5x10(-6) mg/mL for DOPMZ and 4.7x10(-6) mg/mL for DMPMZ. The relative standard deviations were less than 2.8% for ECL intensity and less than 1.1% for migration time. This method was successfully utilized to simultaneously determine PMZ, PMZSO, DOPMZ and DMPMZ in whole urine sample of pet dog.