文献类型：期刊文献

英文题名：Molecular modification of proanthocyanidins

作者：Huo, Qing[1,2];Kong, Xiangye[1];Yang, Xiaofang[1];Wang, Yue[1,2];Ma, Lingling[3,4];Luo, Min[3,4];Xu, Diandou[3,4]

第一作者：Huo, Qing;霍清

通讯作者：Xu, DD[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing, Peoples R China;[3]Chinese Acad Sci, Inst High Energy Phys, Beijing, Peoples R China;[4]Beijing Engn Res Ctr Radiog Tech & Equipment, Beijing, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Chinese Acad Sci, Inst High Energy Phys, Beijing Engn Res Ctr Radiog Tech & Equipment, Key Lab Nucl Radiat & Nucl Energy Technol, Beijing 100049, Peoples R China.

年份：2016

卷号：7

期号：4

起止页码：274-281

外文期刊名：BIOENGINEERED

收录：;Scopus(收录号：2-s2.0-84979536676);WOS:【SCI-EXPANDED(收录号:WOS:000381581400010)】；

基金：This work was financially supported by the 2014 Beijing Natural Science Foundation - Beijing Institute of Science and Technology jointly funded projects L140006, National Natural Science Foundation of China (Grant No. 11475020, 11475198, 11275216 and 11435002), project (No. PXM2016_014209_000023) for building virtual teaching and researching team to enhance teaching quality and project (No. PXM2016_014209_000049) for building emerging major by joint training to enhance the quality of graduate.

语种：英文

外文关键词：esterification; lipase-catalyzed; Novozym435; proanthocyanidins; peroxide value

摘要：Regioselective enzymatic acylation of proanthocyanidin is proposed and investigated as a method by which to improve the solubility of proanthocyanidins in the oil phase and maintain its oxidation resistance. Experimental results indicate that butanol functions as the best solvent in the studied reaction, in which Lipase Novozym435 is used as biological catalyst enzyme and the molar ratio of lauric acid to proanthocyanidins is 4:1. To increase the esterification conversion, we propose the addition of molecular sieve at 5 h. The product was separated by TLC, and results indicate an optimal solvent ratio of ethyl acetate: petroleum ether: acetic acid = 2:3:0.5. This condition can effectively separate the ester and proanthocyanidins, achieving an esterification yield of 60.9%.