文献类型：期刊文献

英文题名：Expression of the TetA gene encoding TetA efflux protein in E. coli contributes to its increased bacterial resistance toward berberine

作者：Li Y.; Cao Z.-T.; Wang X.-Y.; Ge X.-Z.

第一作者：Li Y.

机构：[1]Beijing Key Laboratory of Biomass Waste Resource Utilization, Biochemical Engineering College, Beijing Union University, Beijing, 100023, China

第一机构：北京联合大学生物化学工程学院生物医药系

通讯机构：[1]Beijing Key Laboratory of Biomass Waste Resource Utilization, Biochemical Engineering College, Beijing Union University, Beijing, 100023, China|[11417144]北京联合大学生物化学工程学院生物医药系;[11417]北京联合大学;[1141726]生物化学工程学院;

年份：2018

卷号：20

期号：4

起止页码：374-384

外文期刊名：Journal of Asian Natural Products Research

收录：Scopus(收录号：2-s2.0-85030153013)

语种：英文

外文关键词：bacterial resistance; berberine; EC₅₀; TetA efflux protein; tetracycline

摘要：Berberine (BBR) is a traditional Chinese medicine in various applications due to its antibacterial effect. Here we investigated the increased bacterial resistance of E. coli toward BBR. The median effective concentration (EC50) of BBR against E. coli was increased when TetA efflux protein (TEP) was introduced. Sixty-five percent of the intracellular BBR was expelled and molecular docking demonstrated the intensive interaction of TEP to BBR. Finally, the combined antibacterial experiment identified that BBR acted as an inhibitor of TEP in detoxification of tetracycline. TEP is the first discovered protein that was related to the bacterial susceptibility to BBR. ? 2017 Informa UK Limited, trading as Taylor & Francis Group.