文献类型：期刊文献

英文题名：Optimization of Fermentation and Transcriptomic Analysis of a High-Protein-Producing Galactomyces geotrichum Strain

作者：Sun, Lu[1];Zhang, Na[1,2];Hua, Wei[1,2];Cheng, Yanling[1,2];Mi, Shengquan[1];Zhou, Cheng[1,2]

通讯作者：Mi, SQ[1];Zhou, C[1];Zhou, C[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Beijing Key Lab Utilizat Biomass Wastes, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Utilizat Biomass Wastes, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2026

卷号：15

期号：11

外文期刊名：FOODS

收录：;Scopus(收录号：2-s2.0-105041522647);WOS:【SCI-EXPANDED(收录号:WOS:001790258000001)】；

基金：This research was funded by National Key R&D Program of China (2022YFC3902403), Natural Science Foundation of Beijing Municipality (M8242025), and the Key Project of Beijing Union University.

语种：英文

外文关键词：Galactomyces geotrichum; protein yield; submerged fermentation; functional gene dissection

摘要：The global shortage of protein resources drives the need for sustainable microbial protein production. Galactomyces geotrichum can convert various substrates into high-quality single-cell protein (SCP). In this study, a strain of Galactomyces geotrichum (Gg6) was isolated and identified from cheese. Through single-factor tests and orthogonal array design, optimal fermentation conditions were established, achieving a protein content of 54.56 +/- 0.05% and a protein yield of 6.12 +/- 0.11 g/L in shake flask cultivation (n = 3). Phosphate (PO43-) was identified as the key regulatory ion, and supplementation with potassium dihydrogen phosphate (KH2PO4) significantly enhanced amino acid accumulation. Transcriptomic analysis revealed that KH2PO4 reprograms central carbon metabolism to support amino acid biosynthesis. These findings provide an experimental and theoretical basis for the industrial application and genetic improvement of this strain in microbial protein production.