文献类型：期刊文献

英文题名：Multipath effects of berberine on peach Brown rot fungus Monilinia fructicola

作者：Pei, Qinghui[1,2];Li, Ying[2];Ge, Xizhen[2];Tian, Pingfang[1]

第一作者：Pei, Qinghui

通讯作者：Tian, PF[1];Ge, XZ[2]

机构：[1]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China

第一机构：Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China

通讯机构：[1]corresponding author), Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：116

起止页码：92-100

外文期刊名：CROP PROTECTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000454462900012)】；

基金：This work was supported by Collaborative Innovation Program of Chaoyang District in Beijing (CYXC1712), Innovation Capability Enhancement Program for Universities Governed by Beijing Municipal Commission of Education (No. PXM2015_014209_000010), National Key Research and Development Program of China (No. 2017YFD0201105), National High Technology Research and Development Program (863 Program) (No. 2015AA021003), National Natural Science Foundation of China (No. 21276014, 21476011), National Basic Research Program of China (973 Program) (No. 2012CB725200) and Fundamental Research Funds for the Central Universities (YS1407). We express great appreciation towards Geran Tian from Cornell University for polishing this manuscript.

语种：英文

外文关键词：Monilinia fructicola; Peach; brown rot; Berberine; Transcriptomic analysis

摘要：The brown rot disease caused by fungus Monilinia fructicola severely reduces the yield of peach and other stone fruits. Earlier, we found that berberine (BBR), one of isoquinoline alkaloids, strongly halts the growth of M. fructicola. However, the underlying mechanisms remain unclear. Here we report that BBR inhibits M. fructicola at multiple levels. Scanning electronic microscope (SEM) analysis of BBR-treated M. fructicola revealed morphological abnormality of spores and hyphae. Enzyme activity assay showed BBR's significant inhibition on the activities of enzymes succinate dehydrogenase (SDH), malate dehydrogenase (MDH) and ATPase in mitochondrion, indicating that BBR impedes mitochondrial functions. Furthermore, transcriptomic analysis of BBR-induced M. fructicola revealed that most differentially expressed genes were involved in MAP kinase activity, activation of MAPKK activity, and ADP binding. Overall, these results indicate that BBR inhibits M. fructicola through multipath mechanisms, including destruction on cells, interference of mitochondrial functions, and repression of DNA replication and gene expression.