文献类型：期刊文献

英文题名：Protective Effects of Curcumin on Amyloid-beta-Induced Neuronal Oxidative Damage

作者：Huang, Han-Chang[1,2,3];Chang, Ping[1,3];Dai, Xue-Ling[1,3];Jiang, Zhao-Feng[1,3]

第一作者：Huang, Han-Chang;黄汉昌

通讯作者：Jiang, ZF[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[2]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China;[3]Beijing Union Univ, Coll Arts & Sci, Beijing 100191, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, 197 Beitucheng W Rd, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2012

卷号：37

期号：7

起止页码：1584-1597

外文期刊名：NEUROCHEMICAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000304878200025)】；

基金：This research was supported by Project 31071512 Supported by National Natural Science Foundation of China, Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PHR(IHLB)) [PHR20090514], and Scientific Research Common Program of Beijing Municipal Commission of Education (KM201011417002).

语种：英文

外文关键词：Alzheimer's disease (AD); Amyloid-beta (A beta); Curcumin; Molecular interaction; Oxidative damage

摘要：To investigate the protective effects of curcumin against amyloid-beta (A beta)-induced neuronal damage. Primary rat cortical neurons were cultured with different treatments of A beta and curcumin. Neuronal morphologies, viability and damage were assessed. Neuronal oxidative stress was assessed, including extracellular hydrogen peroxide and intracellular reactive oxygen species. The abilities of curcumin to scavenge free radicals and to inhibit A beta aggregation and beta-sheeted formation are further assessed and discussed. Curcumin preserves cell viability, which is decreased by A beta. The results of changed morphology, released Lactate dehydrogenases and cell viability assays indicate that curcumin protects A beta-induced neuronal damage. Curcumin depresses A beta-induced up-regulation of neuronal oxidative stress. The treatment sequence impacts the protective effect of curcumin on A beta-induced neuronal damage. Curcumin shows a more protective effect on neuronal oxidative damage when curcumin was added into cultured neurons not later than A beta, especially prior to A beta. The abilities of curcumin to scavenge free radicals and to inhibit the formation of beta-sheeted aggregation are both beneficial to depress A beta-induced oxidative damage. Curcumin prevents neurons from A beta-induced oxidative damage, implying the therapeutic usage for the treatment of Alzheimer's disease patients.