文献类型：期刊文献

英文题名：Simultaneous Determination of Three Diterpenoid Alkaloids by HPLC in Unprocessed and Processed Fuzi

作者：Cheng, Hongxia[2];Zeng, Yongchi[3];Fang, Cuifen[1];Cheng, Weiming[1]

第一作者：程红霞

通讯作者：Cheng, WM[1]

机构：[1]Zhejiang Inst Food & Drug Control, Dept Tradit Chinese Med, Hangzhou 310004, Zhejiang, Peoples R China;[2]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[3]Hangzhou Innovat TCM Standarizat Res Inst CO Ltd, Hangzhou 310053, Zhejiang, Peoples R China

第一机构：Zhejiang Inst Food & Drug Control, Dept Tradit Chinese Med, Hangzhou 310004, Zhejiang, Peoples R China

通讯机构：[1]corresponding author), Zhejiang Inst Food & Drug Control, Dept Tradit Chinese Med, 86 Lane,1 Jichang Rd, Hangzhou 310004, Zhejiang, Peoples R China.

年份：2012

卷号：24

期号：6

起止页码：2691-2694

外文期刊名：ASIAN JOURNAL OF CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000305033900070)】；

语种：英文

外文关键词：Fuzi; Diterpenoid alkaloid; HPLC

摘要：An HPLC method was developed for the simultaneous determination of three diterpenoid alkaloids (mesaconitine, aconitine and hypaclionitine) in unprocessed and processed Fuzi. The solid phase was Gemini C18 column with isocratic elution using 0.04 M ammonium acetate-acetonitrile as the mobile phase. The results of methodology all fit the analytical requirements and the recoveries were 95.8-103.41% Quantitative analysis of the three alkaloids showed that the contents of the alkaloids varied significantly. It was the first report that figured out the alkaloids quantitative differences in unprocessed and processed Fuzi and could provide a scientific and technical platform for setting up a quality control standard.