文献类型：期刊文献

中文题名：艾叶中主要化学成分的鉴定及其含量测定研究

英文题名：Study on identification and quantitation of main compounds in Artemisiae Argyi Folium

作者：兰晓燕[1];朱龙波[1,5];黄显章[3];刘大会[4];郝庆秀[1];周利[1];杨健[1];郭兰萍[1];张元[1,2];康利平[1]

第一作者：兰晓燕

机构：[1]中国中医科学院中药资源中心,道地药材国家重点实验室培育基地,北京100700;[2]北京联合大学生物化学工程学院,北京100023;[3]南阳理工学院,河南省张仲景方药与免疫调节重点实验室,河南南阳473000;[4]湖北中医药大学中药资源与中药化学湖北省重点实验室,湖北武汉430065;[5]云南中医药大学,云南昆明650500

第一机构：中国中医科学院中药资源中心,道地药材国家重点实验室培育基地,北京100700

年份：2021

卷号：52

期号：24

起止页码：7630-7637

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划(2020YFC1712700);中国中医科学院科技创新工程项目(CI2021A05206);中央本级重大增减支项目—名贵中药资源可持续利用能力建设(2060302)。

语种：中文

中文关键词：艾叶;UPLC-UV-Q/TOF;绿原酸;新绿原酸;隐绿原酸;异绿原酸A;异绿原酸B;异绿原酸C;咖啡酸;夏佛塔苷;芹菜素;高车前素;棕矢车菊素;异泽兰黄素;5,7,3′-三羟基-6,4′,5′-三甲氧基黄酮;蔓荆子黄素

外文关键词：Artemisia argyi Folium;UPLC-UV-Q/TOF;chlorogenic acid;neochlorogenic acid;cryptochlorogenic acid;isochlorogenic acid A;isochlorogenic acid B;isochlorogenic acid C;caffeic acid;chaffaloside;apigenin;plantain;cypermethrin;isozelan yellow

摘要：目的鉴定艾叶Artemisiae Argyi Folium中主要化学成分的结构并建立其含量测定方法。方法利用UPLC-UVQ/TOF进行艾叶分析条件的优化,在对21批艾叶分析的基础上,确定其中紫外和质谱上主要的色谱峰,利用精确相对分子质量和碎片离子鉴定其结构后,利用对照品比对确定结构;进一步采用Waters Acquity I-ClassTM液相色谱系统,Waters Acquity HSS T3色谱柱(100 mm×2.1 mm,1.8μm),乙腈-0.2%磷酸水为流动相,体积流量0.5 mL/min,梯度洗脱,柱温40℃,PDA检测器,检测波长为326 nm,建立艾叶中主要化学成分的含量测定方法。结果推测鉴定了艾叶中32个主要化合物的结构,利用对照品确定了其中15个化合物的结构,并建立了其中14个化合物(绿原酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、咖啡酸、夏佛塔苷、芹菜素、高车前素、棕矢车菊素、异泽兰黄素、5,7,3′-三羟基-6,4′,5′-三甲氧基黄酮、蔓荆子黄素)的UPLC-UV含量测定方法。14个化合物的分离度良好,稳定性和重复性良好,且线性范围宽(0.5~800.0μg/mL)、线性较好(R2>0.999),平均回收率在94.88%~103.58%。结论建立的艾叶中14个主要化合物的含量测定方法可以全面评价艾叶的质量。
Objective To identify the structures of the main chemical constituents of Artemisiae argyi Folium and establish a method for their content determination.Methods UPLC-UV-Q/TOF was used to optimize the conditions for the analysis of Artemisia argyi.On the basis of the analysis of 21 batches of Artemisia argyi Folium,the main chromatographic peaks on ultraviolet and mass spectra were found.The structures were identified by accurate molecular weight and fragment ions.The structures were determined by comparison of standards.The structures were further determined by UPLC-UV and Waters Acquity I-ClassTM solution.A method for content determination was established by using Waters Acquity HSS T3 column(100 mm×2.1 mm,1.8 um),acetonitrile-0.2%phosphoric acid water as mobile phase,flow rate gradient elution at 0.5 mL/min,the column temperature was 40℃,PDA detector,detection wavelength was 326 nm.Results The structures of the main compounds in 32 of Artemisia argyi Folium were identified,and 15 of them were identified by reference substances.The UPLCUV method for simultaneous determination of 14 compounds(chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acids A-C,caffeic acid,chaffaloside,apigenin,plantain,cypermethrin,and isozelan yellow)in Artemisia argyi Folium were established.The 14 compounds have good separation,stability and repeatability,and wide linear range(0.5—800μg/mL)and good linearity(R2>0.999).The average recovery was 94.88%—103.58%.Conclusion The established method for the determination of 14 main compounds in Artemisia argyi Folium can evaluate the quality of Artemisiae Argyi Folium.