文献类型：期刊文献

中文题名：沉香的质量标志物预测与指纹图谱分析验证

英文题名：Prediction of Quality Markers and Fingerprint Analysis of Aquilariae Lignum Resinatum

作者：杨慧珍[1];和润莹[1];李映[1];葛喜珍[1]

第一作者：杨慧珍

机构：[1]北京联合大学生物化学工程学院,北京100023

第一机构：北京联合大学生物化学工程学院

年份：2022

卷号：39

期号：3

起止页码：285-293

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划项目(SQ2020YFF0400734)

语种：中文

中文关键词：沉香;指纹图谱;网络药理学;质量标志物

外文关键词：Aquilariae Lignum Resinatum;fingerprint;network pharmacology;quality marker

摘要：目的寻找沉香药材的质量标志物。方法通过网络药理学初步筛选出沉香的潜在质量标志物,进一步采用HPLC建立13批不同产地沉香药材的指纹图谱。对13批沉香药材运用主成分分析(principal component analysis,PCA)进行自然聚类,在此基础上,运用正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)筛选不同类别沉香药材的差异性成分,进而验证并推测沉香药材的质量标志物。结果网络药理学预测沉香中的4个活性成分可作为潜在质量标志物。在HPLC指纹图谱共有模式中标定了10个共有峰,指认了沉香四醇峰、6,7-二甲氧基-2-(2-苯乙基)色酮峰和2-(2-苯乙基)色酮峰。PCA与OPLS-DA分析结果表明不同批次沉香药材差异性成分是沉香四醇。同时,结合网络药理学分析结果,确认沉香四醇也是沉香发挥功效的主要活性成分,可作为沉香潜在质量标志物。结论沉香四醇可作为沉香潜在的质量标志物。
OBJECTIVE To find the quality marker(Q-marker)of Aquilariae Lignum Resinatum.METHODS Preliminary screening of potential Q-markers in Aquilariae Lignum Resinatum through network pharmacology,and establishing fingerprints of 13 batches of Aquilariae Lignum Resinatum by HPLC.Principal component analysis(PCA)was used for natural clustering of 13 batches of Aquilariae Lignum Resinatum materials.On this basis,orthogonal partial least squares discriminant analysis(OPLS-DA)was used to screen the different components of different types of Aquilariae Lignum Resinatum materials to verify and speculate Q-marker of Aquilariae Lignum Resinatum.RESULTS Four active ingredients in Aquilariae Lignum Resinatum were predicted as potential Q-markers through network pharmacology.Then ten common peaks were calibrated with HPLC fingerprint.The agarotetrol peak,6,7-dimethoxy-2-(2-phenylethyl)chromone peak and 2-(2-phenethyl)chromone peak were confirmed.The analysis results of PCA and OPLS-DA showed that the differential component of different batches of Aquilariae Lignum Resinatum was agarotetrol.Meanwhile,combined with the results of network pharmacological analysis,it was confirmed that agarotetrol was also the main active component of Aquilariae Lignum Resinatum to exert its efficacy and could be used as a potential Q-marker of Aquilariae Lignum Resinatum.CONCLUSION Agarotetrol can be used as a potential Q-marker of Aquilariae Lignum Resinatum.