文献类型：期刊文献

中文题名：黑果枸杞子对过度训练大鼠骨骼肌MAPK信号通道蛋白表达及抗氧化应激损伤能力的影响

英文题名：Effect of Lycium ruthenicum Fructus on Skeletal Muscle Mitogen Activated Protein Kinase Signaling Pathways Protein Expression and Antioxidantive Stress Damage Capacity in Over-trained Rats

作者：崔笑梅[1];曹建民[2];周海涛[3];王灿[2];万子一[3]

第一作者：崔笑梅

机构：[1]兰州理工大学;[2]北京体育大学;[3]北京联合大学生物化学工程学院

第一机构：兰州理工大学,兰州730050

年份：2017

卷号：23

期号：3

起止页码：122-127

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：北京市朝阳区协同创新项目(CYXC1508);北京市高等学校高水平人才交叉培养-实培计划项目

语种：中文

中文关键词：黑果枸杞子;过度训练;丝裂素活化蛋白激酶信号通路;蛋白表达;抗氧化应激损伤

外文关键词：Lycium ruthenicum fructus; overtraining; mitogen activated protein kinase signaling pathway; protein expression; antioxidantive stress damage

摘要：目的:研究黑果枸杞子对过度训练大鼠骨骼肌MAPK信号通道蛋白表达及抗氧化应激损伤能力的影响。方法:以等量负荷有氧运动训练和大强度耐力训练大鼠为模型,50只SPF级Wistar 49 d龄雄性大鼠为对象,随机分为4组,分别为正常组、有氧训练组、过度训练组、过度训练+黑果枸杞子组,每组12只(剔除不符合实验要求的大鼠)。每天灌胃(ig)给药1次,黑果枸杞子干预组剂量为4.48 mg·kg-1,ig体积为5 m L·kg-1,其他组ig等体积生理盐水。42 d负荷游泳训练后,测试各组大鼠骨骼肌丝裂素活化蛋白激酶(MAPK)信号系统蛋白表达及氧化应激损伤相关指标,分光光度法检测骨骼肌超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;蛋白质免疫印迹(Western blot)法检测MAPK信号通道蛋白表达;酶联免疫吸附测试法(ELISA)检测3-硝基酪氨酸(3-NT),8-羟基脱氧鸟苷(8-OHd G)含量;乙酰半胱氨酸法检测血清肌酸激酶(CK)活性;苯肼显色法检测乳酸脱氢酶(LDH)活性。结果:力竭游泳时间,有氧训练组较正常组有所延长,黑果枸杞子干预组较过度训练组显著增加(P<0.01)。骨骼肌SOD活性,有氧训练组略高于正常组;过度训练组较正常组和有氧训练组明显下降(P<0.05),黑果枸杞子干预组较过度训练组明显升高(P<0.05)。骨骼肌MDA含量,有氧训练组略低于正常组;过度训练组较正常组和有氧训练组显著升高(P<0.01),黑果枸杞子干预组较过度训练组显著降低(P<0.01)。骨骼肌3-NT,8-OHd G,有氧训练组略高于正常组;过度训练组较正常组和有氧训练组显著升高(P<0.01);黑果枸杞子干预组较过度训练组明显降低(P<0.05)。血清LDH活性,有氧训练组略高于正常组;过度训练组较正常组和有氧训练组均显著升高(P<0.01);黑果枸杞子干预组较过度训练组明显升高(P<0.05)。血清CK活性,有氧训练组略高于正常组;过度训练组较正常组和有氧训练组显著升高(P<0.01);黑果枸杞子干预组较过度训练组显著降低(P<0.01)。p38 MAPK,细胞外信号调节蛋白激酶(ERK)信号通路蛋白表达,有氧训练组高于正常组(P<0.05),低于过度训练组(P<0.05);过度训练组高于安静组(P<0.01);黑果枸杞子干预组高于正常组(P<0.05),低于过度训练组(P<0.05)。结论:补充黑果枸杞子可以有效缓解过度训练导致的机体内环境失衡,抑制MAPK信号通路蛋白的过度表达,激活过度训练大鼠骨骼肌MAPK信号转导系统诱导抗氧化酶基因表达,提高机体尤其是骨骼肌的氧自由基清除能力,调节机体的抗氧化应激损伤能力,预防和延缓氧化应激损伤及运动疲劳的发生与发展。
Objective： To study the effect of Lycium ruthenicum fructus on the expression of MAPK signal channel protein and antioxidantive stress damage capacity in skeletal muscle of rats with excessive training.Method： Equivalent load aerobic training and high intensity endurance training rat model was established. A total of 50 49-day old male SPF Wistar rats were randomly divided into 4 groups,with 12 in each group（the rats that did not meet the requirements were removed） ： control group（C Group）,aerobic training group（M group）,overtraining group（OM group）,overtraining and Lycium ruthenicum fructus group（LM group）. The dose of LM group was 4. 48 mg·kg-1,with ig volume of 5 m L·kg-1. The other groups were given equal volume of saline. After42 days ＇ exhaustive swimming training,mitogen activated protein kinase（MAPK） signal channel protein expression and antioxidantive stress damage indicators in skeletal muscle of rats were examined. Spectrophotometry was adopted to detect activity of superoxide dismutase（SOD） and content of malondialdehyde（MDA）. Western blot was used to detect MAPK signal channel protein expression. ELISA was applied in detecting content of 3-nitrotyrosine（3-NT） and 8-hydroxy-2-deoxyguanosine（8-OHd G）. Acetylcysteine method was adopted to detect activity of creatine kinase（CK）. Phenylhydrazine chromogenic method was used to detect activity of lactate dehydrogenase（LDH）. Result： The exhaustive swimming time of M group was longer than C group,and that of LM group was significantly longer than OM group（P 0. 01）. The SOD activity in M group was higher than that in C group,that in OM group was significantly lower than C group and M group（P 0. 05）,and that in LM group was significantly higher than OM group（P 0. 05）. 3-NT and 8-OHd G content in M group was higher than that in C group,that in OM group was significantly higher than C group and M group（P 0. 01）,and that in LM group was significantly lower than OM group（P 0. 01）. The LDH and CK activities of serum in M group was slightly higher than C group,that in OM group was significantly higher than C group and M group（P 0. 01）,and that in LM group was significantly higher than OM group（P 0. 05,P 0. 01）. The expression of p38 MAPK and ERK signal channel protein in M group was higher than that in C group（P 0. 05）,and that in M group was lower than the OM group（P 0. 05）; that in OM group was higher than C group（P 0. 01）; that in LM group was higher than that in C group（P 0. 05）,and that in LM group was lower than OM group（P 0. 05）. Conclusion： The supplementation of Lycium ruthenicum fructus can effectively alleviate the imbalance of the inner environment caused by overtraining,inhibit the excessive expression of MAPK signal channel protein,activate antioxidant enzyme gene expression induced by skeletal muscle MAPK signal transduction system of overtraining rats,improve the capacity of scavenging oxygen free radicals in body,especially skeletal muscle,regulate the antioxidantive stress damage capacity of body,and prevent and delay the oxidative stress injury and the occurrence and development of the sports fatigue.