文献类型：期刊文献

英文题名：Separation of Seven Residues of Fluoroquinolones from Fish Samples using Solid Phase Microextraction and Electrophoresis and their detection by Electrochemiluminescence method

作者：Yang, Fuxiu[1];Zhang, Wenjuan[1];Gu, Chunxiu[1,2];Xu, Jichao[3];Zhou, Kaowen[1]

第一作者：Yang, Fuxiu

通讯作者：Gu, CX[1];Zhou, KW[1];Gu, CX[2];Xu, JC[3]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[3]Qingdao Inst Food & Drug Control, Qingdao 266071, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[3]corresponding author), Qingdao Inst Food & Drug Control, Qingdao 266071, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2020

卷号：15

期号：9

起止页码：9300-9312

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85091506854);WOS:【SCI-EXPANDED(收录号:WOS:000570963900074)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No.2152013), Science and Technology Innovation Project for University Students of Beijing Union University (201911417XJ262) and Postgraduate Funding Project of Beijing Union University (2019-068).

语种：英文

外文关键词：Fluoroquinolones; Fish; Solid phase microextraction; Capillary electrophoresis; Electrochemiluminescence

摘要：A new method for the simultaneous determination of 7 fluoroquinolones (FQs) in fish was established based on purifing and enriching by solid phase microextraction, separating by capillary electrophoresis (CE) and detecting by column end electrochemiluminescence. The effects of pH value, extraction time, extraction temperature, ionic strength and eluate type on the extraction efficiency of 7 FQs were investigated. The effects of buffer additives and separation voltage on the separation efficiency of 7 FQs were investigated. The best purification and enrichment parameters of 10.0 ml extraction solution made from 2.0 g fish sample are: pH value of extraction system is 7, extraction time is 40 min, extraction temperature is 50 degrees C, addition of NaCl is 2.0 g, and eluate is 2.0 ml methanol (containing 5% formic acid). The separation solution of CE is 0.02 mol/L phosphate buffer solution (pH=6, containing 18% cyclodextrin), and the separation voltage is 20 kV. All the 7 FQs have good linear relationship. Their detection limits were 0.4-0.8 mu g/L. The recovery of fish samples was 89.2%-110.4%. 4 FQs were detected in two fish samples. This method has the advantages of small matrix effect, high sensitivity, simplicity and accuracy. It can be used for the detection of FQs in other fish or meat.