文献类型：期刊文献

英文题名：Determination of Four Residues of Fluoroquinolones with Similar Structure in Fish by Modified QuEChERS Protocol Coupled to Capillary Electrophoresis (CE) with End-Column Electrochemiluminescence (ECL)

作者：Zhang, Wenjuan[1];Yang, Fuxiu[1];Wang, Hong[1];Gu, Chunxiu[1,2];Zhou, Kaowen[1,2]

通讯作者：Gu, CX[1];Zhou, KW[1];Gu, CX[2];Zhou, KW[2]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2020

卷号：15

期号：7

起止页码：6802-6814

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85087684963);WOS:【SCI-EXPANDED(收录号:WOS:000551732800065)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No.2152013) and State 863 Program of China (2015AA020200).

语种：英文

外文关键词：Fluoroquinolones; Residue; Fish; CE-ECL; QuEChERS

摘要：A new method was established for simultaneousyl determining ciprofloxacin (CIP), enrofloxacin (ENR), norfloxacin (NOR) and pefloxacin (PEF) in fish by capillary electrophoresis (CE) coupled with end-column electrochemiluminescence (ECL) with modified QuEChERS. The structural differences of 4 FQs are too small to separate them by conventional CE. However, their separation efficiency can be improved by adjusting the composition of separation buffer. The parameters about ECL analysis and CE separation were investigated in detail. The use of methanol and sodium sulfate in QuEChERS can improve the pretreatment effect of the sample. The optimum experimental conditions include analysis conditions (detection potential 1.20 V vs. Ag/AgCl, Ru(bpy)(3)(2+) concentration 6 mmol/L, phosphate buffer solution concentration 40 mmol/L and pH 7.0 in ECL detection cell), separation conditions (separation voltage 13.0 kV, 17.5% cyclodextrin solution (v/v) as additive, phosphate buffer solution concentration 20 mmol/L and pH 5.0 in capillary), and sample conditions (injection time 7 s and injection voltage 12.0 kV). The limits of detection (3 sigma) of this method were 8.8 x 10(-5) mg/mL for CIP, 3.5 x 10(-6) mg/mL for ENR, 7.5 x 10(-5) mg/mL for NOR and 2.7 x 10(-6) mg/mL for PEF. The relative standard deviations (RSD) were less than 2.6% for ECL intensity and less than 2.3% for migration time. This method was successfully utilized to simultaneously determine CIP, ENR, NOR and PEF in fish.