文献类型：期刊文献

英文题名：Cytotoxic steroids from Monascus purpureus-fermented rice

作者：Shang, Xiao-Ya[1];Li, Jin-Jie[1];Liu, Ming-Tao[1,2,3];Li, Shuai[2,3];Liu, Ying[2,3];Wang, Ye-Feng[4,5];Huang, Xiao[4,5];Jin, Zong-Lian[1]

第一作者：尚小雅

通讯作者：Shang, XY[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[2]Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China;[3]Peking Union Med Coll, Beijing 100050, Peoples R China;[4]Beijing Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China;[5]Beijing Univ, Sch Life Sci, Beijing 100871, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, 197 Beitucheng W Rd, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2011

卷号：76

期号：10-11

起止页码：1185-1189

外文期刊名：STEROIDS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000294074700030)】；

基金：Financial support from the Beijing Natural Sciences Foundation (Grant No. 7102020); Scientific Research Common Program of Beijing Municipal Commission of Education (Grant No. KM201111417001) and Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University (Grant No. LD-KZ-0605).

语种：英文

外文关键词：Monascus purpureus; Absolute configuration; X-ray single crystal diffraction; Cytotoxicity

摘要：Bioassay-guided fractionation of an EtOH extract of Monascus purpureus-fermented rice led to the isolation of two new steroids (22S, 23R, 24S)-20 beta,23 alpha,25 alpha-trihydroxy-16,22-epoxy-4,6,8(14)-trienergosta-3-one (1), the first example of a steroid possessing both a conjugated triene ketone system and a fused 4H-furan ring side chain within one molecule, and (22E, 24R)-3 beta,5 alpha-dihydroxyergosta-23-methyl-7,22-dien-6-one (2), as well as two known compounds (22E, 24R)-3 beta,5 alpha-dihydroxyergosta-7,22-dien-6-one (3) and (22E, 24R)-6 beta-methoxy-ergosta-7,22-diene-3 beta,5 alpha-diol (4). Their structures were assigned by detailed interpretation of HRESIMS, 1D and 2D NMR spectroscopic data. The absolute stereochemistry of 1 was determined by single-crystal X-ray crystallography while the absolute stereochemistry of 2 was established by CD. Compounds 1-4 showed cytotoxic activity against the lung adenocarcinoma (A549) with IC(50) values of 0.08, 0.94, 12.6 and 13.5 mu M, respectively. In addition, compounds 1 and 2 exhibited moderate activities against human ovarian cancer (A2780), with IC(50) values of 2.8 and 5.1 mu M. (C) 2011 Elsevier Inc. All rights reserved.