文献类型：会议论文

英文题名：Extraction immunocompetent sections of Agaricus blazei Murill polysaccharides by membranes technology

作者：Xiao, Ye[1,2]; Hongmei, Liu[2]; Hong, Yan[1]; Rugang, Zhong[1,3]

第一作者：Xiao, Ye

通讯作者：Rugang, Z.

机构：[1] Beijing University of Technology, Beijing, 100022, China; [2] Biochemical Engineering College, Beijing Union University, Beijing, 100023, China; [3] College of Life Science and Bioengineering, Beijing University of Technology, No.100 Pingleyuan Chaoyang District, Beijing, 10022, China

第一机构：Beijing University of Technology, Beijing, 100022, China

通讯机构：[3]College of Life Science and Bioengineering, Beijing University of Technology, No.100 Pingleyuan Chaoyang District, Beijing, 10022, China

会议论文集：2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

会议日期：May 23, 2007 - May 27, 2007

会议地点：Beijing, China

语种：英文

外文关键词：Biomedical engineering - Cell proliferation - Gel permeation chromatography - Lymphocytes - Microfiltration - Molecular weight - Polysaccharides

摘要：The bioactive of polysaccharides have relationship with their molecular weight Agarcus blazai Murill (ABM) polysaccharides were extracted by membranes technology, and were graded according to the molecular weight using the combined method of microfiltration (MF), ultrafiltration (UF) and nanofiltration (NF) membrane modules. The effect of gradation were indicated by the high performance gel permeation chromatography (HPGPC), and lymphocyte proliferations in the different molecular weight ABM at the concentration range of 10-800 μ g/ml were detected with MTT method. The results of the experiment indicated that the MF-UF-NF combined method was effective for ABM, and were remarkably shown to increase lymphocyte proliferation at 100000Da of 100, 200, 400, 300μ g/ml ABM (p