文献类型：期刊文献

中文题名：雨生红球藻对过度训练大鼠骨骼肌MAPK信号通道蛋白表达及抗氧化能力的影响

英文题名：Effects of Haematococcus Pluvialis on Mitogen-activated Protein Kinase Signaling Pathways Protein Expression and Antioxidant Capacity in Overtraining Rat Skeletal Muscle

作者：佟强[1];曹建民[2];周海涛[3]

第一作者：佟强

机构：[1]吉林大学体育学院;[2]北京体育大学;[3]北京联合大学生物化学工程学院

第一机构：吉林大学体育学院,吉林长春130012

年份：2016

卷号：52

期号：4

起止页码：133-138

中文期刊名：中国体育科技

外文期刊名：China Sport Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;北大核心:【北大核心2014】；CSSCI:【CSSCI2014_2016】；

基金：北京市朝阳区协同创新项目(CYXC1508)

语种：中文

中文关键词：雨生红球藻;过度训练;丝裂素活化蛋白激酶;蛋白表达;抗氧化

外文关键词：Haematococcus Pluvialis; overtraining; mitogen-activated protein kinase; protein expression; antioxidant

摘要：目的:研究雨生红球藻(Haematococcus Pluvialis)对过度训练大鼠骨骼肌MAPK信号通道蛋白表达及抗氧化能力的影响。方法:以等量负荷有氧运动训练和大强度耐力训练大鼠为模型,50只SPF级Wistar 7周龄雄性大鼠为对象,随机分为安静对照组(C组)、有氧训练组(M组)、过度训练组(OM组)、过度训练+雨生红球藻组(HOM组)4组,每组12只(剔除不符合实验要求的大鼠)。每天灌胃(ig)给药1次,雨生红球藻干预组剂量为0.4mg/kg,ig体积为5mL/kg,其他组ig等体积生理盐水。42d力竭游泳训练后,采用Western Blot法测定各组大鼠骨骼肌丝裂素活化蛋白激酶信号系统(mitogen-activated protein kinase,MAPK)的蛋白表达含量,分光光度法测定骨骼肌SOD活性及MDA含量。结果:有氧训练使M组大鼠力竭游泳时间较C组显著增加(P<0.05);过度训练则使OM组大鼠力竭游泳时间显著低于C组(P<0.05)和M组(P<0.01);而补充雨生红球藻可使HOM组大鼠的力竭游泳时间显著高于OM组(P<0.01),与C组和M组相比则无显著性差异(P>0.05)。过度训练使OM组大鼠骨骼肌超氧化物歧化酶(Superoxide Dismutase,SOD)活性显著低于其他各组(P<0.05);而补充雨生红球藻则使HOM组大鼠SOD显著高于OM组(P<0.05),保持在与C组和M组接近的水平(P>0.05)。过度训练使OM组大鼠骨骼肌丙二醛(Malondialdehyde,MDA)含量较C组和M组明显升高(P<0.01);而补充雨生红球藻则使HOM组的MDA显著低于OM组(P<0.01),且与C组和M组无显著性差异(P>0.05)。有氧训练使M组大鼠p38 MAPK、细胞外信号调节蛋白激酶(extracellular-signal regulatrd protein kinase,ERK)信号通路蛋白表达较C组显著升高(P<0.05);而过度训练则使OM组大鼠p38MAPK、ERK蛋白表达进一步升高,与C组相比有非常显著性差异(P<0.01),与M组相比无显著性差异(P>0.05);补充雨生红球藻使HOM组大鼠p38 MAPK、ERK蛋白表达显著低于OM组(P<0.05),但高于C组(P<0.05)。结论:1)过度训练导致MAPK信号通路蛋白过度表达,氧化-抗氧化系统失衡,氧化应激反应加剧,对机体造成损伤;2)补充雨生红球藻可以抑制MAPK信号通路蛋白的过度表达,提高骨骼肌抗氧化酶的活性和清除自由基的能力,调节机体的抗氧化能力,预防和延缓运动疲劳的发生与发展。
To analyze the effect of Haematococcus Pluvialis on the expression of MAPK signal channel protein and antioxidant capacity in skeletal muscle of rats with overtraining.Method：To establish equivalent load aerobic training and high intensity endurance training rats as a model,50 7-week-old male SPF Wistar rats were randomly divided into 4groups,with 12 in each group（the rats that did not meet the requirements were removed）：control group（C Group）,aerobic training group（M group）,overtraining group（OM group）,overtraining and Haematococcus Pluvialis group（HOM group）.The dose of HOM group was 0.4 mg/kg,the volume was 5 ml/kg.Other groups was given equal volume of saline.After 42 days swimming training,mitogen activated protein kinase（MAPK）signal channel protein expression and antioxidant indicators（SOD and MDA）in skeletal muscle of rats were evaluated by western blot and spectrophotometry.Results：The exhaustive swimming time of M group was longer than C group（P〉0.05）,and that of HOM group was significantly longer than OM group（P〈0.01）.The SOD activity in M group was higher than that in C group（P〉0.05）;and that in OM group was significantly lower than C group and M group（P〈0.05）,and that in HOM group was significantly higher than OM group（P〈0.05）.The MDA content in M group was slightly lower than C group（P〈0.05）;that in OM group was significantly higher than C group and M group（P〉0.01）,that in HOM group was significantly lower than OM group（P〈0.01）.The expression of p38 MAPK and ERK signal channel protein in M group was higher than that in C group（P〈0.05）,and that in M group was lower than the OM group（P〈0.05）;that in OM group was higher than C group（P〈0.01）;that in LM group was higher than that in C group（P〈0.05）,and that in HOM group was lower than OM group（P〈0.05）.Conclusions：1）The overtraining leads to an excessive expression of MAPK signal channel protein,and an imbalance of oxidation and antioxidation system,causes oxidative stress and damage of the body.2）The supplementation of Haematococcus Pluvialisafter Murr.can inhibit the excessive expression of MAPK signal channel protein,and activate the MAPK signal channel so as to regulate the expression of antioxidant genes,and improve the capacity of scavenging oxygen free radical in skeletal muscle,and regulate the antioxidant capacity of body,and prevent and delay the occurrence and development of exercise fatigue.