文献类型：期刊文献

英文题名：HPLC Fingerprint of Solid-State Fermentation of Fugui Gutong Compound Prescription

作者：Su, Jianshu[1,2];Tian, Pingfang[2];Lin, Qiang[1];Liu, Baining[1];Zheng, Laili[1,2];Ge, Xizhen[1]

第一作者：Su, Jianshu

通讯作者：Ge, XZ[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2013

卷号：25

期号：6

起止页码：3063-3067

外文期刊名：ASIAN JOURNAL OF CHEMISTRY

收录：;Scopus(收录号：2-s2.0-84875184322);WOS:【SCI-EXPANDED(收录号:WOS:000317245900028)】；

基金：This study was supported by a grant from the Beijing Municipal Education Commission of the People's Republic of China (KM200911417011).

语种：英文

外文关键词：Aspergillus niger; Fu-Gui-Gu-Tong; Solid state fermentation; HPLC fingerprint

摘要：Aspergillus ruger (E6.22) was employed for solid state fermentation of Fu-Gui-Gu-Tong (FGGT, hereafter solid state fermentation FGGT), a notable Chinese compound prescription, to attenuate its toxicity. Results indicated that the optimal spore density was 1.8 x 10(4) mL(-1), incubation time was 7d, pH was 6.5, incubation temperature was 30 degrees C. Under these conditions, paeoniflorin and total alkaloids were increased, but aconitine was decreased. Additionally, high-performance liquid chromatographic fingerprint of solid state fermentation Fu-Gui-Gu-Tong were determined and applied for monitoring the fermentation stability. The similarity index of 10 samples was 0.978, suggesting that the procedure of solid state fermentation Fu-Gui-Gu-Tong was stable in different batches. To investigate its acute toxicity, the mice was orally administered with graded doses of solid state fermentation Fu-Gui-Gu-Tong extract the LD50 of the solid state fermentation Fu-Gui-Gu-Tong group was higher than Fu-Gui-Gu-Tong, meaning the less toxicity of solid state fermentation Fu-Gui-Gu-Tong. Hence, it is concluded that A. niger (E6.22) could alleviate the toxicity of Fu-Gui-Gu-Tong through solid state fermentation and the fermentation procedure was reliable and applicable.