文献类型：期刊文献

英文题名：Tetrahydroisoquinoline N-methyltransferase from Methylotenera Is an Essential Enzyme for the Biodegradation of Berberine in Soil Water

作者：He, Runying[1];Cui, Yao[1];Li, Ying[1];Ge, Xizhen[1]

第一作者：He, Runying

通讯作者：Li, Y[1];Ge, XZ[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2022

卷号：27

期号：17

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000851851200001)】；

基金：This research was funded by Major project for the transformation of scientific and technological achievements in Hebei Province [19026517Z]; Beijing Municipal Commission of Education [KZ201911417049].

语种：英文

外文关键词：berberine; soil water; Methylotenera; tetrahydroisoquinoline N-methyltransferase; biodegradability

摘要：Berberine (BBR), a Chinese herbal medicine used in intestinal infection, has been applied as a botanical pesticide in the prevention of fungal disease in recent years. However, its degradation in the environment remains poorly understood. Here, we investigated BBR's degradation in soil water from different sources accompanied by its effect on bacterial diversity. Our results indicated that BBR was only degraded in soil water, while it was stable in tap water, river water and aquaculture water. Bacterial amplicon results of these samples suggested that the degradation of BBR was closely related to the enrichment of Methylotenera. To reveal this special relationship, we used bioinformatics tools to make alignments between the whole genome of Methylotenera and the pathway of BBR's degradation. An ortholog of Tetrahydroisoquinoline N-methyltransferase from plant was discovered only in Methylotenera that catalyzed a crucial step in BBR's degradation pathway. In summary, our work indicated that Methylotenera was an essential bacterial genus in the degradation of BBR in the environment because of its Tetrahydroisoquinoline N-methyltransferase. This study provided new insights into BBR's degradation in the environment, laying foundations for its application as a botanical pesticide.