文献类型：期刊文献

英文题名：Cost-effective optimization of gellan gum production by Sphingomonas paucimobilis using corn steep liquor

作者：Huang, Jin[1];Zhu, Shengquan[1];Li, Chuanbao[1];Zhang, Chenglin[2];Ji, Yizhi[3]

第一作者：Huang, Jin

通讯作者：Huang, J[1]

机构：[1]Zhejiang Univ Technol, Coll Pharmaceut Sci, Chaowang Rd 18, Hangzhou 310014, Zhejiang, Peoples R China;[2]Tianjin Engn Res Ctr Microbial Metab & Fermentat, Tianjin, Peoples R China;[3]Beijing Union Univ, Coll Biochem & Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing, Peoples R China

第一机构：Zhejiang Univ Technol, Coll Pharmaceut Sci, Chaowang Rd 18, Hangzhou 310014, Zhejiang, Peoples R China

通讯机构：[1]corresponding author), Zhejiang Univ Technol, Coll Pharmaceut Sci, Chaowang Rd 18, Hangzhou 310014, Zhejiang, Peoples R China.

年份：2020

卷号：50

期号：2

起止页码：191-197

外文期刊名：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000497033300001)】；

基金：The project was supported by the Yunhe Program of Zhejiang University of Technology, the General Project of Beijing Municipal Education Commission (No. SQKM201311417004) and the foundation (No.ZXKF20180204) of Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, P.R. China.

语种：英文

外文关键词：Gellan gum; CSL; cost-effective; Plackett-Burman design; Box-Behnken

摘要：Gellan gum, produced by Sphingomonas paucimobilis, is increasingly used in food and pharmaceutical industries as stabilizing, emulsifying, texturing and gelling agents. However, its high production costs may limit its full commercial potential. Therefore, in this study, we investigated ways to reduce gellan gum production costs and improve yields. We first revealed corn steep liquor (CSL) as a cost-effective nutrient source that can improve gellan gum yields. We then systematically optimized culture conditions even further, and revealed that the addition of Triton X-100 surfactant and selected inorganic nitrogen sources improved gellan gum production. Under our optimized conditions (glucose 33.75 g/L, CSL 10 g/L, urea 2.5 g/L, MgSO4 1.08 g/L, KH2PO4 3.24 g/L, K2SO4 1 g/L and Triton X-100 0.75 g/L), we yielded a maximum concentration of 14.41 g/L, which was about 1.5-fold higher than non-optimized CSL-based medium. Our findings highlight the use of CSL as a cost effective and promising nutrient source for industrial production of gellan gum.