文献类型：期刊文献

英文题名：Successive Extraction of Glycyrrhizic Acid and Liquiritin from Licorice with Cellulase and Pectinase

作者：Ge, Xizhen[2];Lin, Qiang[2];Gu, Chunxiu[2];Zhu, Yan[2];Jiang, Nan[2];Zhao, Bingbing[2];Wang, Meng[2];Tian, Pingfang[1]

第一作者：葛喜珍

通讯作者：Tian, PF[1]

机构：[1]Beijing Inst Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China;[2]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China

第一机构：Beijing Inst Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

通讯机构：[1]corresponding author), Beijing Inst Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China.

年份：2012

卷号：24

期号：10

起止页码：4487-4490

外文期刊名：ASIAN JOURNAL OF CHEMISTRY

收录：;Scopus(收录号：2-s2.0-84861672703);WOS:【SCI-EXPANDED(收录号:WOS:000310771000056)】；

基金：This study was supported by Science and Technology Development Project of Beijing Educational Committee, China (KM200911417011, KM200611417013).

语种：英文

外文关键词：Licorice; Liquiritin; Glycyrrhizic acid; Cellulose; Pectinase; Extract successively

摘要：A simple and environment amicable method for successive extraction of glycyrrhizic acid (GA) and liquiritin from licorice root was developed using cellulase and pectinase for pretreatment (licorice root: cellulose: pectinase = 650:2:1, pH 5.0-6.0, 50 degrees C, 160-180 mm). 10 % ammonia as a solvent was verified to be more effective than 10 % ethanol for extracting glycyrrhizic acid from licorice based on pretreatment with pectinase and cellulose, resulting in 45.61 % of glycyrrhizic acid purity. Subsequently, 10 and 60 % of aqueous ethanol solution were recruited to successively elute glycyrrhizic acid from the enzyme-digested extracts in D101 macroporous adsorption resin (MAR) and the glycyrrhizic acid purity was increased to 86.33 %. After extraction of glycyrrhizic acid, the residue of licorice root was treated with the above two kinds of enzymes, extracted with 90% ethanol, 0.32% of liquiritin purity was achieved. Eluted by macroporous adsorption resin AB-8, the purity of liquiritin was up to 2.78 %. Purified by polyamide, the purity was elevated to 10.89 %. Compared with previous protocols, this method is benign to the environment and the employed enzymes and macroporous resin are suitable for large-scale separation of glycyrrhizic acid and liquiritin from licorice root successively.