文献类型：期刊文献

中文题名：五步蛇蛇毒的分离纯化及综合利用

英文题名：Isolation and Comprehensive Utilization of Enzymes from Agkistrodon acutus Venom

作者：付大雁[1];赵伟[2];郭锴[1];俞军[2]

第一作者：付大雁

机构：[1]北京化工大学教育部超重力工程研究中心;[2]北京联合大学生物化学工程学院

第一机构：北京化工大学教育部超重力工程研究中心,北京100029

年份：2005

卷号：25

期号：7

起止页码：98-99

中文期刊名：中国生物工程杂志

外文期刊名：Progress In Biotechnology

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：综合利用;分离纯化;五步蛇;蛇毒;SDS-PAGE图谱;Sephadex;阴离子交换层析;类凝血酶;酶分子量;纤溶酶;回收率;DEAE;HPLC;开发利用;冻干粉;分子筛;活力

外文关键词：Fibrinolytic enzyme Thrombin-like enzyme Isolation Comprehensive utilization

摘要：五步蛇蛇毒冻干粉经过SephadexG-75分子筛层析,使纤溶酶和类凝血酶初步分离;DEAE阴离子交换层析对2种酶进一步分离纯化,分别得到了纤溶酶和类凝血酶。2种酶在HPLC图谱上均呈单一峰,在SDS-PAGE图谱上均为单一条带,纤溶酶分子量大约为24.1kDa,类凝血酶分子量大约为14.4kDa,与以往报道相符。酶的总活力回收率大大提高,纤溶酶的活力回收率达23.9%,类凝血酶的活力回收率达34.5%。实现了对蛇毒的综合利用,为进一步开发利用蛇毒探索了一条有效的途径。
The Agkistrodon acutus venom was fractionated by chromatographies with Sephadex G-75, DEAE, respectively. The result of the experiment shows that the purified enzymes are single peeks on C18 HPLC and single bands on SDS-PAGE. The molecular mass of fibrinolytic enzyme is 24.1 kDa, and that of thrombin-like Enzyme is 14.4 kDa. The special activities of the two different enzymes purified were definitely recovered. The activity recoveries for each one are greatly improved to 23.3% (fibrinolytic enzyme) and 34.5% (thrombin-like Enzyme) respectively. Fibrinolytic enzyme and Thrombin-like Enzyme were both isolated from Agkistrodon acutus venom, therefore it is an efficient approach for the comprehensive utilization of the venom.