文献类型：期刊文献

中文题名：姜黄素对D-半乳糖致衰老大鼠氧化应激及Nrf2/ARE通路的影响

英文题名：Effects of curcumin on the oxidative stress and Nrf2/ARE pathway involves the attenuating in aging rats injected with subcutaneous D-galactose

作者：郭豫[1];赵建[1];赵江燕[1];姜招峰[1,2];张冬雪[1,2];黄汉昌[1,2]

第一作者：郭豫

机构：[1]北京联合大学功能食品科学技术研究院;[2]北京联合大学应用文理学院

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院功能食品科学技术研究院

年份：2016

卷号：31

期号：3

起止页码：232-234

中文期刊名：华西药学杂志

外文期刊名：West China Journal of Pharmaceutical Sciences

收录：CSTPCD;;CSCD:【CSCD2015_2016】；

基金：国家自然科学基金资助项目(批准号:31471587);北京市属高等学校高层次人才引进与培养三年行动计划(2013-2015年)青年拔尖人才培育计划资助项目(CIT&TCD201504034)

语种：中文

中文关键词：姜黄素;D-半乳糖;氧化应激;Nrf2;抗氧化应激元件;衰老;抗氧化;蛋白表达

外文关键词：Curcumin; D-galactose; Oxidative stress; Nrf2; Antioxidantresponse element; Aging; Antioxidant; Protein expression

摘要：目的研究姜黄素对D-半乳糖致衰老模型大鼠氧化应激及Nrf2/ARE通路的影响。方法将大鼠随机均分为空白组、模型组和姜黄素组于模型组和姜黄素组大鼠每日sc 125 mg·kg-1D-半乳糖造模,姜黄素组大鼠同时ip 10 mg·kg-1姜黄素,空白组大鼠给予生理盐水,连续7周。测定各组大鼠血清中丙二醛(MDA)、肝组织中蛋白质糖基、谷胱甘肽过氧化物(GSH)的含量和血清超氧化物歧化酶(SOD)、全血谷胱甘肽过氧化物酶(GSH-Px)的活性;用Western blot法分析肝组织中Nrf2蛋白和HO-1蛋白的表达水平。结果与模型组比较,姜黄素组大鼠血清中SOD的活性和MDA、肝中GSH、蛋白质羰基的含量均降低,全血中GSH-Px的活性显著升高,Nfr2和HO-1蛋白的表达水平也明显升高。结论姜黄素能够缓解D-半乳糖导致的大鼠氧化应激,Nrf2/ARE通路参与了姜黄素的抗氧化活性。
OBJECTIVE To investigate the effects of curcumin on the oxidative stress and activity of transcription factor Nrf2 and antioxidant response element（ARE） in aging rats treated with subcutaneous D-galactose.METHODS SD rats were randomly divided into blank group,model group and curcumin group.Rats in model group and curcumin group were subcutaneously injected with125 mg·kg-1D-galactose daily for 7 weeks.Rats in curcumin group were also sc curcumin 10 mg·kg-1daily.Blank group rats were administrated vehicle solution.Content of serum malondialdehyde（MDA）,activity of superoxide dismutase（SOD） in the serum and glutathione peroxidase（GSH-Px） in blood,protein carbonyl and GSH content in the liver were detected.Further,the protein expression levels of Nrf2 and heme oxygenase-1（HO-1） were analyzed by Western bolt method.RESULTS Compared with the model group,SOD activity in the serum decreased and the content of MDA,protein carbonyl and GSH in the liver decreased,but the blood GSH-Px activity increased in curcumin group.Further,the protein expression levels of Nrf2 and HO-1 in the liver were also up-regulated in the curcumin group,compared with the model group.CONCLUSION Curcumin can relieve the oxidative stress in the rats resulted from subcutaneous D-galactose,and Nrf2/ARE pathway may involve in the physiological function on curcumin-mediated antioxidation.