文献类型：期刊文献

中文题名：海娇颜片中原花青素含量快速测定方法优化

英文题名：Optimization of rapid content determination method of procyanidin in Haijiaoyan tablets

作者：闫文杰[1,2];李凯[1];吕倩倩[1];焦丽娟[2];刘倩[1];王志鑫[1,2]

第一作者：闫文杰

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]北京联合大学保健食品功能检测中心,北京100191

第一机构：北京联合大学生物化学工程学院

年份：2020

卷号：0

期号：3

起止页码：841-846

中文期刊名：食品安全质量检测学报

外文期刊名：Journal of Food Safety & Quality

收录：CSTPCD

基金：北京市教育委员会科技计划一般项目(KM201811417003).

语种：中文

中文关键词：原花青素;海娇颜片;铁盐催化比色法;大孔树脂;乙醇沉淀-离心法

外文关键词：procyanidin;Haijiaoyan tablets;colorimetry with ferric ions as catalysis agent;macroporous resin;ethanol precipitation-centrifugation

摘要：目的优化快速测定海娇颜片中原花青素含量的分析方法。方法通过加标回收率法发现采用铁盐催化比色法测定海娇颜片中原花青素时的干扰成分,并分别采用大孔树脂柱及乙醇沉淀-离心2种方法对样品进行前净化处理。结果发现海娇颜片中鱼胶原蛋白肽对原花青素的测定存在较大干扰,优化前测得原花青素含量为1.22%,经大孔树脂柱净化后测得其含量为2.15%,而经乙醇沉淀-离心净化后测定其含量为3.11%,接近理论值。结论乙醇沉淀-离心法能显著去除干扰成分,提高原花青素含量测定的准确度,是较优的样品预处理方法。该研究可为相关产品中原花青素含量快速测定方案的制定提供参考依据。
Objective To optimize the method for rapid determination of procyanidin content in Haijiaoyan tablets.Methods The method of spiked recovery was firstly applied to discover the components which had interference on the determination of procyanidin in Haijiaoyan tablets by the typical method of ferric ions as catalyst,and two process methods including macroporous resin column and ethanol precipitation-centrifugation were then applied to pretreat the sample.Results Fish collagen peptide in Haijiaoyan tablets had obvious interference on the determination of procyanidin.The detected content of procyanidin was 1.22%before optimization,2.15%after purification through macroporous resin column,and 3.11%after the treatment by ethanol precipitation-centrifugation,respectively.And the last one was much closer to the theoretical value.Conclusion Ethanol precipitation-centrifugation method could significantly remove the interfering components and improve the accuracy of determination for procyanidin,which is the better strategy for sample pretreatment.This study could provide reference for the protocol establishment of rapid determination of procyanidin in health products.