文献类型：期刊文献

英文题名：Biogas Efficiency and Microbial Community Characteristics in Thermophilic Anaerobic Digestion of Kitchen Waste

作者：Xie, Yufeng[1];Qiang, Jingwen[1];Tang, Manyu[1];Zhou, Ziang[1];Wang, Xinyi[1];Wang, Wanqing[1];Wu, Shuang[1];Zhang, Na[1];Hua, Wei[1];Zhou, Cheng[1];Cheng, Yanling[1]

第一作者：Xie, Yufeng

通讯作者：Cheng, YL[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2026

卷号：19

期号：14

外文期刊名：ENERGIES

收录：;EI(收录号：20263021190799);Scopus(收录号：2-s2.0-105045680206);WOS:【SCI-EXPANDED(收录号:WOS:001831735400001)】；

基金：The authors are grateful for the financial support provided by the National Key R&D Program of China (2022YFC3902403), the Natural Science Foundation of Beijing Municipality (M8242025), and the Key Project of Beijing Union University.

语种：英文

外文关键词：kitchen waste; biogas slurry; methane; inoculation ratio; methanogenic archaea

摘要：Anaerobic digestion of kitchen waste (KW) for biogas production has significant promise both economically and environmentally. However, low biogas production and fluctuating methane concentration remain key challenges that need to be overcome to make this a viable solution. This study implements a two-stage evaluation framework to link temperature-dependent microbial characteristics with process optimization. First, high-throughput sequencing was performed across a wide temperature gradient (25 degrees C, 37 degrees C, 45 degrees C, 50 degrees C, 55 degrees C, and 60 degrees C) to characterize the baseline microbial community screening and succession trajectories of methanogenic archaea. Based on the identified thermophilic transition threshold, five batch anaerobic reactors were subsequently configured under the target thermophilic condition (50 +/- 1 degrees C) with substrate-to-inoculum (S/I) ratios calculated on a volatile solids (VS) basis (1:0, 1.5:1, 1:1, 1:1.5, and 1:2) to evaluate the biomethane potential, organic removal rates, and volatile fatty acid (VFA) conversion. The results showed that an optimal S/I ratio of 1:1 was crucial for maximizing methane production performance, achieving the highest cumulative biogas yield of 1494.07 mL/g VS and methane yield of 746.28 mL/g VS. Notably, Methanoculleus and Candidatus Methanoplasma were identified as the core functional methanogens enriched at high-temperature stages. Both genera are obligate hydrogenotrophic methanogens that consume H-2 and CO2 as substrates, and their enrichment plays a vital syntrophic role in alleviating intermediate acid accumulation and maintaining steady methanogenesis. This study provides clear theoretical insights and experimental data supporting the efficient thermophilic utilization of KW via precise microbial load balancing.