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HPLC测定紫草软胶囊中左旋紫草素的含量    

Determination of content of shikonin from Radix lithospermi soft capsule by HPLC

文献类型:期刊文献

中文题名:HPLC测定紫草软胶囊中左旋紫草素的含量

英文题名:Determination of content of shikonin from Radix lithospermi soft capsule by HPLC

作者:蔡玫[1];张元[2]

第一作者:蔡玫

机构:[1]海南省药品检验所;[2]北京联合大学生物化学工程学院

第一机构:海南省药品检验所,海南海口570216

年份:2008

卷号:8

期号:8

起止页码:1443-1444

中文期刊名:中国热带医学

外文期刊名:China Tropical Medicine

收录:CSTPCD

语种:中文

中文关键词:紫草软胶囊;左旋紫草素;HPLC;含量测定

外文关键词:Radix arnebiae soft capsule; Shikonin; HPLC ; Determination content

摘要:目的建立紫草软胶囊中左旋紫草素的含量测定方法。方法采用HPLC法,考证于不同流动相组合、流速及柱温等条件下测定紫草软胶囊中左旋紫草素的效果。结果以DIKMAODS C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水-甲酸(85:15:0.5)为流动相,流速1.0ml·min^-1,波长516nm,柱温25℃,进样量100μl。检测紫草软胶囊中左旋紫草素在10.2-35.3μg·ml^-1浓度范围内线形关系良好(r=0.9997);平均回收率为100.6%,RSD=1.83%(n=5)。结论所建立的HPLC实验方法简便、检测结果准确,可用于紫草软胶囊的质量控制和评价。
Objective To establish a method for the determination of content of shikonnin in Radix arnebiae soft capsules by HPLC. Methods The content of shikonin was determined by HPLC, the chromatographic conditions were DIKMAODS C18 column ( 5μm, 250mm×4.6mm), mobile phase methanol - water - Formic Acid ( 85 : 15 : 0.5 ), detection wavelength 516 nm, temperature 25 ℃, flow rate 1.0ml·min^-1 with the load of 100μl. Results It keeps a good linear when the shikonin was 10.2-35.31μg·ml^-1 (r=0.9997), and the average recovery rate was 100.6 % , RSD =1.83%. Conclusion This method was a simple, accurate one with a good reproducibility that can be used for the quality control in assessmen of the quality of Radix amebiae soft capsule.

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