文献类型：期刊文献

中文题名：水溶性有机试剂萃取杜氏藻中的β-胡萝卜素

英文题名：Extraction of β-Carotene from Dunaliella salina by Water-Soluble Organic Solvent

作者：惠伯棣[1];许岱[1];宫平[1];高洁[1]

第一作者：惠伯棣

机构：[1]北京联合大学应用文理学院食品科学系

第一机构：北京联合大学生物化学工程学院食品科学系|北京联合大学应用文理学院

年份：2015

卷号：0

期号：10

起止页码：179-184

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

语种：中文

中文关键词：杜氏藻;β-胡萝卜素;水溶性有机溶剂;酶;几何异构;高效液相色谱

外文关键词：Dunaliella salina;;β-carotene;;water-soluble organic solvent;;enzyme;;geometrical isomer;;HPLC

摘要：使用水溶性有机溶剂建立适用于杜氏藻中β-胡萝卜素萃取的方法。以乙酸乙酯为参比,用丙酮、甲醇、乙醇和四氢呋喃4种水溶性有机溶剂,对实验室养殖的杜氏藻体在室温条件下分别进行萃取。用C18-高效液相色谱(high performance liquid chromatography,HPLC)和C30-HPLC分别测定萃取物中β-胡萝卜素含量及其几何异构体的组成。通过比较不同溶剂萃取的β-胡萝卜素含量和所消耗的溶剂体积,筛选出适合食品工业生产中使用的水溶性萃取溶剂。在进一步考虑各溶剂的食品安全性之后,确定乙醇为最适溶剂。使用乙醇作为萃取溶剂,能够从每毫克(干质量)藻体中萃取得到β-胡萝卜素0.32μg。其中,总顺式异构体的比例占20.21%(m/m)。随后,使用4种碳水化合物和1种蛋白质水解酶对藻体的细胞壁和膜进行破坏。观察原料酶解对β-胡萝卜素萃取量和溶剂消耗的影响。实验结果表明,原料酶解可导致其中β-胡萝卜素的损失,并对溶剂消耗无明显改进,不建议采用。
This study aimed to develop a method for β-carotene extraction from Dunaliella salina by water-soluble organic solvents. In experiments, in reference to ethyl acetate, four water-soluble organic solvents, namely acetone, methanol, ethanol and tetrahydrofuran, were selected as media to extract β-carotene from Dunaliella salina cultured under laboratory conditions. β-carotene and its geometrical isomers in each extract were determined by C18-HPLC and C30-HPLC, respectively. By comparing the extraction efficiency of β-carotene and solvent consumption and meanwhile considering food safety, ethanol was selected as the most suitable medium for industrial practice. By using ethanol as the extraction medium, 0.32 μg of β-carotene was extracted from 1 mg of dried Dunaliella salina with 20.21%(m/m) of total Z-isomers. Four carbohydrates and one protease were then employed to destroy the cell wall and membrane of algae. The influences of enzymatic hydrolysis of raw material on the extraction efficiency of β-carotene and solvent consumption were observed. Data obtained from this investigation suggested that enzymatic hydrolysis of Dunaliella salina resulted in loss of β-carotene amount and exhibited no improvement in solvent consumption. It is therefore not considered to be applied.