文献类型：期刊文献

英文题名：Surface decoration with leucine tetrapeptide: An antibacterial strategy against Gram-negative bacteria

作者：Xu, Dandan[1,2];Ju, Xiaoyan[1];Zhu, Meng[1];Ou, Jinzhao[1,2];Lu, Guojun[1,2];Wan, Chenxiao[1,2];Li, Kejia[1,2];Jiang, Wei[1,2];Jia, Xinyu[3];Han, Yongping[3];Tian, Ye[1,5];Niu, Zhongwei[1,4,5]

第一作者：Xu, Dandan

通讯作者：Tian, Y[1];Niu, ZW[1]

机构：[1]Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[4]Univ Chinese Acad Sci, Sch Future Technol, Beijing 100049, Peoples R China;[5]Chinese Acad Sci, Tech Inst Phys & Chem, Beijing, Peoples R China

第一机构：Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China

通讯机构：[1]corresponding author), Chinese Acad Sci, Tech Inst Phys & Chem, Beijing, Peoples R China.

年份：2023

卷号：641

起止页码：126-134

外文期刊名：JOURNAL OF COLLOID AND INTERFACE SCIENCE

收录：;EI(收录号：20231113735543);Scopus(收录号：2-s2.0-85149829725);WOS:【SCI-EXPANDED(收录号:WOS:000955975800001)】；

基金：This work was supported by the National Natural Science Foundation of China (52073293 and 31971303), the Cross Training Plan for High Level Talents in Beijing, the Innovation Special Foundation for High Level Research Institutions in Zhongshan (2019AG003) and the Presidential Foundation of Technical Institute of Physics and Chemistry. We thank Prof. Jingxin Meng and Prof. Liping Wen at Technical Institute of Physics and Chemistry, Chinese Academy of Sciences for their help in the contact angle analysis and surface zeta potential measurement.

语种：英文

外文关键词：Peptide; Anti-bacteria; Surface coating; Lipopolysaccharide; Gram-negative bacteria

摘要：Surface-associated microbe contamination by Gram-negative bacteria poses a serious problem in medical care. Cationic peptides or polymers are the main materials used for antibacterial surface coating, but the positive charge may lead to blood coagulation. Therefore, exploiting surface coating which is free of pos-itive charge and is effective for Gram-negative bacteria inactivation is in urgent need. In this study, inspired by the affinity between lipopolysaccharides of Gram-negative bacteria and Toll-like receptors of immune cells, we develop a leucine-based tetrapeptide coating strategy for combating Gram-negative bacteria. The obtained surface has excellent bactericidal activity against Gram-negative bacteria like Pseudomonas aeruginosa and Escherichia coli. A 1 mm2 coated glass surface could kill > 9.9 x 104 CFU bacteria in 1 h and has nearly no damage to mammal cells. Moreover, this surface coating strategy could be applied on various surfaces like glass slices, glass capillary cavity and thermoplastic polyurethane slices. And the coated surface could largely mitigate the microbe contamination in an in vivo subcuta-neous implantation. This work paves a new way for antibacterial surface-coating which is behaving no positive charge and is of great importance for biomedical devices.(c) 2023 Elsevier Inc. All rights reserved.