文献类型：期刊文献

中文题名：小檗碱壳聚糖微球制备及其抗真菌活性测定

英文题名：Preparation of Berberine-loaded Chitosan Microspheres and Their Fungistasis

作者：侯东耀[1];葛喜珍[2];刘军锋[1];田平芳[1];谭天伟[1]

第一作者：侯东耀

机构：[1]北京化工大学生命科学与技术学院,北京100029;[2]北京联合大学生物化学工程学院,北京100023

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2008

卷号：8

期号：5

起止页码：962-966

中文期刊名：过程工程学报

外文期刊名：The Chinese Journal of Process Engineering

收录：CSTPCD;;EI(收录号：20084711718530);Scopus(收录号：2-s2.0-55949107129);北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：小檗碱;壳聚糖;微球;抗真菌活性

外文关键词：berberine; chitosan; microsphere; fungistasis

摘要：采用乳化-化学交联法制备了负载小檗碱的壳聚糖微球.以正交实验对微球形态、粒径、药物包封率和载药率等指标进行了制备工艺条件优化.显微镜和电镜观察显示微球球形良好,表面光滑,平均粒径约15μm,包封率为78.98%,载药率为4.78%.持续30d的药物释放实验表明,小檗碱可从微球中缓慢释放.利用生长速率法测定了微球对3种重要植物病原真菌的抑制作用,5μg/mL微球对番茄早疫病菌(Alternaria solani)的抑菌率达65%.
Berberine-loaded chitosan microspheres were prepared by emulsion-chemical crosslinking technique. The preparation conditions were optimized by orthogonal experimental design with respect to particle size distribution, embedding rate and drug loading. The prepared microspheres showed perfect spherical geometry, smooth surface and suitable size distribution while observed under optic and scanning electron microscopes. The average diameter of microspheres was 15 p.m, with drug loading of 4.78% and embedding rate of 78.98%. Subsequently, their drug release in vitro was tested in the phosphate buffered saline （pH 6.8） for 30 d, and the release curve demonstrated an appropriate speed. In addition, inhibitory rates of the microspheres against Alternaria solani, Colletotrichum capsici and Botrytis cinerea were determined using growth rate method at microsphere concentration of 5μg/mL. Exhilaratingly, the microspheres showed 65% inhibitory rate against Alternaria solani, one predominant pathogen causing post-harvest disease of tomato.