文献类型：期刊文献

英文题名：Hydroxypropyl beta-cyclodextrin improving multiple stresses tolerance of Lactococcus lactis subsp. lactis

作者：Cui, Lianming[1];Lin, Songyang[1];Yi, Juanjuan[1];Liu, Xin[1];Hao, Limin[2];Ji, Yizhi[3];Lu, Laizheng[1];Ji, Zhenyu[5];Kang, Qiaozhen[1];Lu, Jike[4]

第一作者：Cui, Lianming

通讯作者：Hao, LM[1]

机构：[1]Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China;[2]Acad Mil Sci PLA China, Quartermaster Equipment Inst, Beijing 100010, Peoples R China;[3]Beijing Union Univ, Coll Biochem & Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[4]Zhengzhou Mindtek Biotechnol Co Ltd, Zhengzhou 450001, Henan, Peoples R China;[5]Zhengzhou Univ, Inst Med & Pharmaceut Sci, Zhengzhou 450001, Henan, Peoples R China

第一机构：Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China

通讯机构：[1]corresponding author), Acad Mil Sci PLA China, Quartermaster Equipment Inst, Beijing 100010, Peoples R China.

年份：2020

卷号：85

期号：7

起止页码：2171-2176

外文期刊名：JOURNAL OF FOOD SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000536495000001)】；

基金：This study was supported by the National Natural Science Foundation of China (81870093), the Research Project of People's Liberation Army (BX115C007), the Training Project for Young Outstanding Teachers of University in Henan Province (2017GGJS0090), the Opening Foundation of Beijing Key Laboratory of Biomass Waste Resource Utilization.

语种：英文

外文关键词：hydroxypropyl beta-cyclodextrin; Lactococcus lactis subsp; Lactis; protective agent; stress response

摘要：L. lactis is known as industrial starter in the fermentation of dairy and meat products, and it plays an important role in human health as an edible probiotic. During industrial production, L. lactis often experiences different stresses that delay the growth and decrease the survival in some serious conditions. In this study, the protective effects of hydroxypropyl beta-cyclodextrin (HP beta-CD) on L. lactis under multiple stresses were investigated. The microbial cells were treated with different stresses including heat, NaCl, cold, and H2O2 stresses, and the results were showed by measuring the OD600 or spot plating method. The growth and tolerance were improved when HP beta-CD was added during different stress conditions, better than that of trehalose. Besides, the scanning electron microscopic and fluorescence spectrum studies showed that HP beta-CD could combine with L. lactis to protect the cell structure, suggesting that HP beta-CD may act as a protective agent of L. lactis. Therefore, HP beta-CD could be considered as a potential protective agent to be applied in food industry, and its protective mechanism on L. lactis still needs further investigation.