文献类型：期刊文献

英文题名：Taurine attenuates lipid accumulation via the eCB-CB1 axis: evidence from adipose metabolomics in HFD-fed mice and 3D adipocyte spheroids

作者：Wu, Qingjie[1,2];Shao, Yuxin[1,2];Zhu, Lin[1,2];Guo, Xinzhe[1,2];Mi, Shengquan[1,2];Zhang, Yanzhen[1,2];Chang, Ping[2];Xie, Changying[2];Guo, Junxia[1,2]

第一作者：Wu, Qingjie

通讯作者：Guo, JX[1];Guo, JX[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Food, Beijing, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[2]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Food, Beijing, Peoples R China.|[114172]北京联合大学应用文理学院;[11417]北京联合大学;[1141726]北京联合大学生物化学工程学院;

年份：2026

卷号：13

外文期刊名：FRONTIERS IN NUTRITION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001718895800001)】；

基金：The author(s) declared that financial support was received for this work and/or its publication. This research was funded by the Academic Research Projects of Beijing Union University, grant number ZK10202206.

语种：英文

外文关键词：cannabinoid receptor type 1; endocannabinoid; lipid accumulation; obesity; taurine

摘要：Introduction Obesity, driven by adipose tissue dysfunction, is a major global health challenge and a key contributor to metabolic disorders. Although taurine shows anti-obesity potential, its precise mechanisms for attenuating adipocyte lipid accumulation remain unclear.Methods In this study, high-fat diet (HFD)-induced obese mice were treated orally with taurine (700 mg/kg/day) for 14 weeks. Systemic obesity-related parameters were evaluated, with a focus on epididymal white adipose tissue (eWAT). UPLC-MS-based metabolomics combined with multivariate analysis was employed to characterize metabolic alterations in eWAT. Additionally, 3T3-L1 adipocyte spheroids were treated with taurine (0-0.5 mM), either alone or in combination with the cannabinoid receptor type 1 (CB1) agonist CP55940 or antagonist AM6545, to assess its effects on lipid accumulation and underlying mechanisms.Results Focusing on adipose tissue, taurine treatment effectively countered HFD-induced metabolic disturbances, particularly by suppressing epididymal fat mass accumulation and ameliorating adipocyte hypertrophy. Metabolomic profiling of eWAT revealed that taurine treatment reversed 15 out of 35 metabolic alterations, including the reduction of three anandamide (AEA) precursors, implying that taurine may alter endocannabinoid (eCB) biosynthesis by limiting precursor availability. Moreover, taurine suppressed lipid accumulation by inhibiting CB1 signaling, a mechanism supported by downregulation of lipogenic genes (including Srebf1, Acaca, Cd36, and Pparg) and upregulation of lipolytic genes (including Pnpla2, Lipe, and Ppargc1a).Conclusion Collectively, our findings demonstrate that taurine exerts its anti-obesity effects partially via modulation of eCB-CB1 signaling, coordinately inhibiting lipogenesis and promoting lipolysis, thereby highlighting its therapeutic potential for obesity management.