文献类型：期刊文献

中文题名：纤维素酶降解结合膜法制备低聚壳聚糖

英文题名：Preparation of Oligochitosan by Cellulase Depolymerization with Membrane Technology

作者：韩永萍[1];林强[1]

第一作者：韩永萍

机构：[1]北京联合大学生物化工学院

第一机构：北京联合大学生物化学工程学院

年份：2007

卷号：48

期号：2

起止页码：98-101

中文期刊名：化学世界

外文期刊名：Chemical World

收录：北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：壳聚糖;低聚壳聚糖;降解;纤维素酶;超滤

外文关键词：chitosan; oligochitosan; depolymerization; cellulase; ultrafiltration

摘要：研究纤维素酶降解结合膜法制备低聚壳聚糖。采用正交设计优选酶降解的最佳工艺条件及分析超滤产品的平均相对分子质量和收率。纤维素酶对壳聚糖降解的最佳条件为:温度50℃,酶糖质量比为0.1,pH=4.6。纤维素酶催化降解结合膜法制备低聚壳聚糖。在5 h内即可获得收率超过75%,平均相对分子质量在5000左右的优质低聚壳聚糖产品。该工艺简单可行,运行时间短,产品相对平均分子质量小而且分布窄,收率较高。
Chitobiose was prepared by cellulase depolymerization with membrane technology. The cellulase depolymerization conditions were optimized by orthogonal test design. The average molecular weight and yield of ultrafiltrated product was analyzed. The optimum cellulase depolymerization conditions were： temperature 50 ℃ ; the mass of cellulase to chitosan 0.1 and pH = 4.6. The oligochitosan with molecular mass about 5000 was obtained by cellulase depolymerization with membrane technology within 5 h and the recovery rate was about 75 %. The process was simple and feasible, the product had a feature of low average relative molecular mass and narrow distribution and the recovery was high.