文献类型：期刊文献

英文题名：Simultaneous Extraction of Polysaccharides and Isoflavone from Semen sojae Preparatum and Assay for Anti-alpha-Glucosidase Activity

作者：Ge, Xizhen[2];Zhang, Jing[1,2];Lin, Qiang[2];Tian, Pingfang[1]

第一作者：葛喜珍

通讯作者：Tian, PF[1]

机构：[1]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China;[2]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China

第一机构：Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

通讯机构：[1]corresponding author), Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China.

年份：2012

卷号：24

期号：4

起止页码：1671-1674

外文期刊名：ASIAN JOURNAL OF CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000300464200059)】；

基金：This study was supported by Science and Technology Development Project of Beijing Educational Committee, China (KM200911417011, KM200611417013).

语种：英文

外文关键词：Semen sojae preparatum; Polysaccharides; Isoflavone; Anti-alpha-glucosidase

摘要：Isoflavone from Semen sojae preparatum (ISSP) was extracted with 80 % ethanol by ultrasonic method. The extract was subjected to macroporous resin AB-8, eluted by 80 % ethanol and analyzed with HPLC. The main isoflavone included genistin (1076.80 mu g/g), daidzein (910.22 mu g/g) and genistein (1128.61 mu g/g). Subsequently, polysaccharides from Semen sojae preparatum (PSSP) present in sediments were extracted with distilled water using ultrasonic method, the total crude polysaccharides accounted for 13.3 mg/g, which were further purified, two of them, PSSPI and PSSPII manifested molecular weight of 9500 and 7000 Da, respectively. By establishment of in vitro alpha-glucosidase inhibitor models, the inhibitory rate of ISSP to alpha-glucosidase was shown to be 40.6%, while both PSSPI and PSSPII exhibited anti-alpha-glucosidase activity of 3 %.