文献类型：期刊文献

英文题名：The Synergism of Human Lactobacillaceae and Inulin Decrease Hyperglycemia via Regulating the Composition of Gut Microbiota and Metabolic Profiles in db/db Mice

作者：Li, Peifan[1];Tong, Tong[1];Wu, Yusong[1];Zhou, Xin[1];Zhang, Michael[2,3];Liu, Jia[4];She, Yongxin[5];Li, Zuming[1];Li, Yongli[6]

第一作者：Li, Peifan

通讯作者：Li, ZM[1];Li, YL[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Univ Manitoba, Dept Phys & Astron, Winnipeg, MB R3T 2N2, Canada;[3]Sino Canada Hlth Engn Res Inst, Hefei, Peoples R China;[4]Internal Trade Food Sci & Technol Beijing Co Ltd, Beijing 102209, Peoples R China;[5]Chinese Acad Agr Sci, Inst Qual Standard & Testing Technol AgroProd, Beijing, Peoples R China;[6]Zhengzhou Univ, Henan Prov Peoples Hosp, Peoples Hosp, Zhengzhou, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]corresponding author), Zhengzhou Univ, Henan Prov Peoples Hosp, Peoples Hosp, Zhengzhou, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2023

卷号：33

期号：12

起止页码：1657-1670

外文期刊名：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001166324900012)】；

基金：This work was supported by the [Natural Science Foundation of Beijing Municipality] under Grant [6173033]; [Academic Research Projects of Beijing Union University] under Grant [ZK70202003]; [Sino Canada health engineering research institute cooperation projects] under Grant [50304JA1901]; [Internal trade food science and technology (Beijing) Co., Ltd cooperation projects] under Grant [202116].

语种：英文

外文关键词：Human Lactobacillaceae; type 2 diabetes mellitus; inulin; gut microbiota; metabolomics

摘要：This study aimed to evaluate the effects of Limosilactobacillus fermentum and Lactiplantibacillus plantarum isolated from human feces coordinating with inulin on the composition of gut microbiota and metabolic profiles in db/db mice. These supplements were administered to db/db mice for 12 weeks. The results showed that the Lactobacillaceae coordinating with inulin group ( LI) exhibited lower fasting blood glucose levels than the model control group (MC). Additionally, LI was found to enhance colon tissue and increase the levels of short-chain fatty acids. 16S rRNA sequencing revealed that the abundance of Corynebacterium and Proteus, which were significantly increased in the MC group compared with NC group, were significantly decreased by the treatment of LI that also restored the key genera of the Lachnospiraceae_NK4A136_ group, Lachnoclostridium, Ruminococcus_ gnavus_group, Desulfovibrio, and Lachnospiraceae_UCG- 006. Untargeted metabolomics analysis showed that lotaustralin, 5-hydroxyindoleacetic acid, and 13(S)-HpODE were increased while L-phenylalanine and L-tryptophan were decreased in the MC group compared with the NC group. However, the intervention of LI reversed the levels of these metabolites in the intestine. Correlation analysis revealed that Lachnoclostridium and Ruminococcus_gnavus_ group were negatively correlated with 5-hydroxyindoleacetic acid and 13(S)-HpODE, but positively correlated with L-tryptophan. 13(S)-HpODE was involved in the "linoleic acid metabolism". L-tryptophan and 5-hydroxyindoleacetic acid were involved in "tryptophan metabolism" and "serotonergic synapse". These findings suggest that LI may alleviate type 2 diabetes symptoms by modulating the abundance of Ruminococcus_gnavus_group and Lachnoclostridium to regulate the pathways of "linoleic acid metabolism", "serotonergic synapse", and" tryptophan metabolism". Our results provide new insights into prevention and treatment of type 2 diabetes.