文献类型：期刊文献

中文题名：葡萄籽原花青素对顺铂诱导小鼠睾丸支持细胞TM4细胞凋亡的影响

英文题名：Effect of Grape Seed Proanthocyanidin Extract on Cisplatin-Induced Apoptosis of Mouse Testicular Sertoli TM4 Cells

作者：杜月梅[1,2];韩何丹[1,2];郭卓雨[1,2];高丽萍[1,2]

第一作者：杜月梅

通讯作者：Gao, Liping

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]生物活性物质与功能食品北京市重点实验室,北京100191

第一机构：北京联合大学生物化学工程学院

年份：2020

卷号：41

期号：9

起止页码：98-104

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;EI(收录号：20202508857838);Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：北京市自然科学基金项目(7163211);生物活性物质与功能食品北京市重点实验室开放课题(LDSP201801)。

语种：中文

中文关键词：葡萄籽原花青素;顺铂;小鼠睾丸支持细胞;细胞凋亡

外文关键词：grape seed proanthocyanidin extract;cisplatin;mouse testicular sertoli TM4 cells;cell apoptosis

摘要：目的:研究葡萄籽原花青素(grape seed proanthocyanidin extract,GSPE)对顺铂(cisdichlorodiamineplatinum(Ⅱ),cDDP)诱导小鼠睾丸支持细胞(TM4)细胞凋亡、细胞凋亡形态及凋亡相关基因蛋白表达的影响。方法:体外培养正常TM4细胞,将实验细胞分为空白对照组、cDDP组(0.014 mg/mL)、GSPE(0.005 mg/mL)+cDDP组(0.014 mg/mL)、GSPE组(0.005 mg/mL)。流式细胞检测法测定GSPE对cDDP诱导TM4细胞凋亡率的影响,Hoechst染液染色法测定TM4细胞凋亡的形态学变化,Western blot蛋白免疫印迹法测定凋亡相关基因蛋白表达情况。结果:cDDP可显著增加TM4细胞凋亡,显著降低TM4细胞Bcl-2表达量以及处于酶原状态Pro-caspase3表达量,而明显增加TM4细胞Bax表达量,同时增加处于激活状态的Caspase-3表达量。GSPE可明显降低cDDP诱导的TM4细胞凋亡,降低Bax以及Caspase-3表达量,增加Bcl-2和Pro-caspase3表达量。结论:细胞凋亡在cDDP诱导TM4细胞毒性过程中起重要作用。Bcl-2、Bax、Caspase-3等蛋白参与cDDP诱导TM4细胞凋亡,GSPE通过抑制Bax及Caspase-3的蛋白表达及增强Bcl-2和Pro-caspase3的表达来阻抑cDDP诱导TM4细胞的凋亡,从而减轻c DDP诱导TM4细胞损伤。
Objective:To study the effect of grape seed procyanidin extract(GSPE)combined with cisplatin(cisdichlorodiamineplatinum(Ⅱ),cDDP)on cell apoptosis and apoptosis-related protein expression in mouse testicular sertoli TM4 cells.Methods:Normal TM4 cells were cultured in vitro and divided into control,cDDP(0.014 mg/mL),GSPE(0.005 mg/m L)+cDDP(0.014 mg/mL)and GSPE(0.005 mg/mL)group.Flow cytometry was used to determine the effect of GSPE on cDDP-induced cell apoptosis rate,Hoechst staining was used to determine the morphological change of TM4 cells,and Western blot was used to determine the protein expression levels of apoptosis-related genes.Results:cDDP significantly increased the apoptosis of TM4 cells,significantly decreased the expression of the anti-apoptosis proteins Bcl-2 and pro-caspase 3,significantly increased the expression of the pro-apoptosis protein Bax,and increased the expression of activated caspase-3.GSPE could significantly reduce cDDP-induced apoptosis,reduce the expression of Bax and caspase-3,and increase the expression of Bcl-2 and pro-caspase3.Conclusion:Apoptosis plays an important role in TM4 cytotoxicity induced by cDDP.Bcl-2,Bax,caspase-3 and other proteins are involved in the cDDP-induced apoptosis of TM4 cells,which can be inhibited by GSPE through inhibiting the protein expression of Bax and caspase-3 and enhancing the expression of Bcl-2 and pro-caspase 3,thereby reducing cDDP-induced damage to TM4 cells.