文献类型：期刊文献

英文题名：Design, application and verification of a novel system utilizing bacteria and microalgae to treat swine farm wastewater and produce value-added biomass

作者：Zhen, Xin[1];Li, Yali[1];Qiang, Jingwen[1];Tang, Manyu[1];Hua, Wei[1,6];Wang, Wanqing[1,2];Wu, Shuang[1,2];Perkins, Phil[3];Ruan, Roger[4,5];Cheng, Yanling[2,4,5,7]

通讯作者：Hua, W[1];Cheng, YL[2];Hua, W[3];Cheng, YL[4]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[3]Solaster LLC, Nashville, TN 37210 USA;[4]Univ Minnesota, Ctr Biorefining, St Paul, MN 55108 USA;[5]Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA;[6]18 Sanqu, Beijing 100023, Peoples R China;[7]Beijing Union Univ, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[3]corresponding author), 18 Sanqu, Beijing 100023, Peoples R China;[4]corresponding author), Beijing Union Univ, Beijing 100023, Peoples R China.|[11417]北京联合大学;[1141726]北京联合大学生物化学工程学院;

年份：2023

卷号：16

期号：4

起止页码：222-230

外文期刊名：INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING

收录：;Scopus(收录号：2-s2.0-85175044752);WOS:【SCI-EXPANDED(收录号:WOS:001089339900001)】；

基金：The authors acknowledge that this work was financially supported by National Key R&D Program of China (Grant No. 2022YFC3902403), Beijing excellent talent training project (Grant No. 2018000020144G074), Special project on green campus of Beijing Union University (Grant No. zk50202101), Research innovation grant for graduate students of Beijing Union University (Grant No. yz2020k001), Scientific research project grants from Beijing Union University (Grant No. ZK130202103, JB202101, XP202004), University of Minnesota Mn Drive Environment (MNE12) and Center for Biorefining.

语种：英文

外文关键词：bacteria-microalgae; swine wastewater; environmental impact; value-added biomass; biofertilizer

摘要：Swine farm wastewater is extremely harmful to the environment if not treated before it is discharged. In this study, a system was developed and optimized for testing the high levels of organic matter in swine farm wastewater utilizing a microalgae/bacteria co-culture combined with a novel closed-loop extraction and dilution process. Importantly, the system produces biomass that also could be harvested and used in value-added applications. The efficacy of biomass as a biofertilizer was demonstrated by using a model plant of Arabidopsis. In addition, the analysis of biomass indicates that it also has potential as a source for biofuel. After a 20-d cultivation period, a yield of biomass was achieved to 2.063 g /L of wastewater. The highest removal rates recorded in steady state conditions were: 13.8 mg/L center dot d of Total Nitrogen (TN); 11.5 mg/L center dot d of Ammonia Nitrogen ( -N); 24.8 mg/L center dot d of Chemical Oxygen Demand (COD); and 16.9 mg/L center dot d of Total Phosphorus (TP). After cultivation, the composition of the biomass was analyzed on a dry basis; the major components were protein (44.9%), lipids (24.6%), carbohydrates (19.9%), Chlorophyll-A (2.75%), Chlorophyll-B (1.66%), and carotenoids (0.57%). This biomass was diluted with water (5% by weight) and used as a biofertilizer to grow Arabidopsis. The results showed that the average root and stem lengths of Arabidopsis were 43.0% and 55.0% longer compared to those of the control group. Additionally, the number of leaves and the maximum leaf length increased by 30.2% and 39.7%; and the fresh and dry leaf weights increased by 44.0% and 33.7%, respectively. These results demonstrate the efficacy of this system for treating swine farm wastewater whilst simultaneously producing a value-added microalgae/bacteria biomass. This paper also demonstrated the use of biomass as a fertilizer for cultivating a value-added crop and, based on the compositional analyses, propose that the biomass could be used as a raw material for biofuel production due to its high lipid content of 24.6%. By constructing a microalgae/bacteria symbiosis system, Swine farm wastewater can be treated as resources utilizing producing value-added biomass with demonstrated efficacy as a biofertilizer.