文献类型：期刊文献

英文题名：Effects of fruits and vegetables on gut microbiota in a mouse model of metabolic syndrome induced by high-fat diet

作者：Yu, Congcong[1];Guo, Cang[1,2];Geng, Xueying[1];Yao, Yuyang[1];Guo, Junxia[1,3];Zhang, Yanzhen[1,3];Zhang, Jing[1,3];Mi, Shengquan[1,3]

第一作者：Yu, Congcong

通讯作者：Mi, SQ[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[2]Changping Lab, Beijing, Peoples R China;[3]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：0

外文期刊名：FOOD SCIENCE & NUTRITION

收录：;Scopus(收录号：2-s2.0-85141506380);WOS:【SCI-EXPANDED(收录号:WOS:000879100800001)】；

基金：National Key Research and Development Program, Grant/Award Number: 2018YFC131140

语种：英文

外文关键词：C57BL/6N mice; fruits and vegetables; gut microbiota; metabolic syndrome

摘要：The aim of this study was to evaluate the effect of fruit and vegetable intake on gut microbiota using a mouse model of metabolic syndrome (MS) induced by a high-fat diet. Forty-eight male mice were randomly divided into four groups, control group (C), high-fat diet-fed model group (H), high fat plus low intake of fruits and vegetables diet-fed group (H.LFV), high fat plus high intake of fruits and vegetables diet-fed group (H.HFV), and each group were fed for 60days. During the experiment, mouse body weights were recorded and fecal samples were collected. Cetyltrimethyl ammonium bromide (CTAB) method was used to extract fecal bacterial DNA, and the purity and concentration of the DNA were detected by electrophoresis. DNA samples underwent PCR amplification (primers in 16S V4 (515F and 806R)). Raw sequencing data were processed, and sample complexity and multiple-sample comparisons were investigated. Mouse organ coefficient, serum lipid levels, fecal TC (total cholesterol) and TBA (total bile acid) levels, and hepatic glutathione and malondialdehyde levels were determined. Compared to the H group, the fecal TC and TBA levels decreased significantly in the H.HFV group (p <.05), and hepatic glutathione and malondialdehyde levels decreased significantly in both H.LFV and H.HFV groups (p <.05). Decreased abundance of Firmicutes, Burkholderiales, Syntrophomonas, and Pseudomonadales in gut microbiota was observed in H.LFV and H.HFV groups compared to the H group. The Anosim results showed significant differences in pairwise comparison between groups. The linear discriminant analysis effect size (LEfSe) results showed that k_bacteria not only exhibited statistically differences between H and C groups but also among H.LFV, H.LFV, and H groups, and hence, could be used as a biomarker between groups. To sum up, fruit and vegetable powder could increase the fecal excretion of TC and TBA, and the antioxidant capacity in C57BL/6N mice. Meanwhile, the mechanism that fruit and vegetable powder could prevent MS in C57BL/6N mice was related to the decreased abundance of gut microbiota, including Firmicutes, Syntrophomonadales, and Pseudomonadales. Hence, fruit and vegetable powder could be used as a recommended food to regulate gut microbiota and prevent the occurrence of MS-related diseases.