详细信息
姜黄素复合有氧运动对非酒精性脂肪肝大鼠肝功能的改善作用 ( EI收录)
Curcumin Combined with Aerobic Exercise Improved Liver Function in Rats with Non-alcoholic Fatty Liver Disease
文献类型:期刊文献
中文题名:姜黄素复合有氧运动对非酒精性脂肪肝大鼠肝功能的改善作用
英文题名:Curcumin Combined with Aerobic Exercise Improved Liver Function in Rats with Non-alcoholic Fatty Liver Disease
作者:胡戈[1];秦菲[2];曹建民[3];吉喆[4];周绮云[2];周海涛[5]
第一作者:胡戈
机构:[1]常州大学体育学院,江苏常州213164;[2]北京联合大学生物化学工程学院,北京100023;[3]北京体育大学运动人体科学学院,北京100084;[4]安徽师范大学体育学院,安徽芜湖241000;[5]北京联合大学体育运动与健康研究所,北京100101
第一机构:常州大学体育学院,江苏常州213164
年份:2025
卷号:46
期号:10
起止页码:178-187
中文期刊名:食品科学
外文期刊名:Food Science
收录:;EI(收录号:20252118492241);北大核心:【北大核心2023】;
基金:北京联合大学科研项目(ZK30202104)。
语种:中文
中文关键词:姜黄素;有氧运动;腺苷酸活化蛋白激酶/哺乳动物雷帕霉素靶蛋白信号通路;自噬;非酒精性脂肪肝
外文关键词:curcumin;aerobic exercise;adenosine monophosphate-activated protein kinase/mammalian target of rapamycin signaling pathway;autophagy;non-alcoholic fatty liver disease
摘要:目的:研究姜黄素与有氧运动相结合对非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)大鼠肝功能改善的效果及机制。方法:将雄性SD大鼠随机分为对照组、NAFLD模型组、有氧运动干预组、姜黄素干预组和姜黄素复合有氧运动干预组,每组8只。利用全自动生化仪测定大鼠血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartateaminotransferase,AST)活性、总胆固醇(totalcholesterol,TC)、总甘油三酯(total triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇浓度;通过苏木精-伊红、油红O和电子染色法观察大鼠肝组织形态、脂质沉积及超微结构变化;采用实时荧光定量聚合酶链式反应、免疫组化和蛋白免疫印迹法测定大鼠肝腺苷酸活化蛋白激酶(adenosine monophosphateactivated protein kinase,AMPK)、磷酸化腺苷酸活化蛋白激酶(phosphorylated adenosine monophosphate-activated proteinkinase,p-AMPK)、哺乳动物雷帕霉素靶蛋白(mammaliantargetofrapamycin,mTOR)、Beclin-1、选择性自噬接头蛋白p62、微管相关蛋白1轻链3-I(microtubule-associated protein 1 light chain 3-I,LC3-I)、LC3-II mRNA和蛋白表达水平。结果:8周高脂饮食成功诱导大鼠出现NAFLD;姜黄素和/或有氧运动干预可改善NAFLD大鼠肝超微结构损伤、病理变化及脂质沉积;降低血清TC、TG、LDL-C浓度及AST和ALT活性;上调Beclin-1、LC3-II mRNA表达水平及p-AMPK、Beclin-1、LC3-II蛋白表达水平和p-AMPK/AMPK、LC3-II/LC3-I蛋白表达水平比值,下调m TOR、p62 m RNA表达水平和m TOR、p62蛋白表达水平,提升细胞自噬水平;姜黄素与有氧运动在血清TC和TG浓度、ALT和AST活性、肝m TOR、Beclin-1、p62 m RNA表达水平、p-AMPK、p62、LC3-II蛋白表达水平及LC3-II/LC3-I蛋白表达水平比值间存在交互效应。结论:姜黄素和/或有氧运动干预通过调控AMPK/mTOR信号通路适度增强细胞自噬水平,改善NAFLD大鼠肝脏结构和功能,其中,姜黄素复合有氧运动干预效果最佳。
Objective:To explore the effect and mechanism of curcumin combined with aerobic exercise in improving liver function in rats with non-alcoholic fatty liver disease(NAFLD).Methods:Male Sprague Dawley rats were randomly divided into five groups:control,NAFLD model,aerobic exercise intervention,curcumin intervention,and combined intervention with curcumin and aerobic exercise,with 8 rats in each group.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),total triglyceride(TG),low-density lipoprotein-cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were detected using an automatic biochemistry analyzer.Changes in hepatic histomorphology,lipid deposition and ultrastructure were detected by hematoxylin-eosin,oil red O,and electronic staining.The mRNA and protein expression levels of hepatic adenosine monophosphate-activated protein kinase(AMPK),phosphorylated adenosine monophosphate-activated protein kinase(p-AMPK),mammalian target of rapamycin(mTOR),Beclin-1,sequestosome-1 protein(SQSTM1/p62),microtubule-associated protein 1 light chain 3-I(LC3-I),and LC3-II were detected by real-time quantitative polymerase chain reaction(real-time PCR),immunohistochemistry and Western blot.Results:NAFLD was successfully induced after eight weeks of a high-fat diet.Curcumin and/or aerobic exercise intervention ameliorated liver ultrastructural damage,pathological changes,and lipid deposition in NAFLD rats,decreased serum levels of TC,TG,LDL-C,as well as serum AST and ALT activities,up-regulated the mRNA expression levels of hepatic Beclin1 and LC3-II and the protein expression levels of p-AMPK,Beclin1,and LC3-II,along with the protein expression ratios of p-AMPK/AMPK and LC3-II/LC3-I,and down-regulated the mRNA and protein expression levels of mTOR and p62,thereby enhancing cellular autophagy.Curcumin and aerobic exercise interacted with each other to regulate serum TC and TG levels,ALT and AST activities,hepatic mTOR,Beclin-1,and p62 mRNA expression,p-AMPK,p62,and LC3-II protein expression and the protein expression ratio of LC3-II/LC3-I.Conclusion:Curcumin and/or aerobic exercise intervention could improve liver structure and function in NAFLD rats by moderately enhancing cellular autophagy through the regulation of the AMPK/mTOR signaling pathway.The effect of curcumin combined with aerobic exercise was more pronounced than that of either treatment alone.
参考文献:
正在载入数据...