文献类型：期刊文献

中文题名：蜂花粉中总黄酮和SOD酶的测定

英文题名：Measurement of the total-flavonoid and SOD activity in the bee pollen

作者：韩薇妍[1];魏超[1];赵有玺[1];龚平[1]

第一作者：韩薇妍

机构：[1]北京联合大学生物化学工程学院生物医药系

第一机构：北京联合大学生物化学工程学院生物医药系

年份：2009

期号：7

起止页码：257-259

中文期刊名：食品科技

外文期刊名：Food Science and Technology

收录：北大核心:【北大核心2008】；

基金：北京市教委科技面上项目(KM200811417007)

语种：中文

中文关键词：蜂花粉;总黄酮;SOD酶;邻苯三酚

外文关键词：bee pollen; total-flavonoid; super-oxide-dismutase; pyrogallol

摘要：用分光光度法和邻苯三酚自氧化法测定粗花粉、花粉酶解浓缩液以及花粉酶解沉淀物的总黄酮和超氧化物歧化酶(SOD酶)活性。结果:粗花粉总黄酮515.520mg/100g、花粉酶解浓缩液总黄酮1083.074mg/100g、花粉酶解沉淀物总黄酮1256.147mg/100g。花粉酶解浓缩液和花粉酶解沉淀物总黄酮之和是粗花粉总黄酮的4.54倍;SOD酶活力有较高的保留。
The activity of SOD and total-flavonoid, existing in raw materials of bee pollens, concentrated liquid of enzymolysis and deposit after enzymolysis, were measurated by spectrophotometry and pyrogallol autooxidation. It indicated that total-flavonoid of raw materials, concentrated liquid, and deposit after enzymolysis were 515.520, 1083.074, and 1256.147 mg/100 g, respectively. The sum of total-flavonoid of concentrated liquid and deposit after enzymolysis was 4.54 times as much as that of raw materials. The activity of SOD in the concentrated liquid was remained to some extent.