文献类型：期刊文献

中文题名：振腹法对寒凝证类痛经大鼠子宫体表投影温度及前列腺素E2、前列腺素F2α的影响

英文题名：Effect of abdominal vibration on the projection temperature of uterus surface and prostaglandin E_(2) and prostaglandin F_(2α) of cold coagulation dysmenorrhea rats

作者：耿楠[1];胡慧[1];谢丹丹[1];赵思雨[1];吴凡[2];沈潜[1]

第一作者：耿楠

机构：[1]北京中医药大学东方医院,北京100078;[2]北京联合大学特殊教育学院,北京100075

第一机构：北京中医药大学东方医院,北京100078

年份：2022

卷号：19

期号：12

起止页码：4-7

中文期刊名：中国医药导报

外文期刊名：China Medical Herald

收录：CSTPCD

基金：国家自然科学基金资助项目(81904315);中国残疾人联合会课题项目(2020&WT001)。

语种：中文

中文关键词：寒凝证类痛经模型;红外热成像;前列腺素F_(2α);前列腺素E_(2);振腹法

外文关键词：Cold coagulation dysmenorrhea model;Infrared thermal imaging;Prostaglandin F2α;Prostaglandin E2;Abdominal vibration

摘要：目的探讨振腹法对寒凝证类痛经大鼠子宫体表投影温度及前列腺素E_(2)、前列腺素F_(2α)的影响。方法应用随机数字表法将30只SD雌性6周龄、体重(230±20)g大鼠分为对照组、模型组、振腹组,每组10只。模型组和振腹组采用冷冻结合皮下注射苯甲酸雌二醇、腹腔注射缩宫素联合致痛进行模型制备,对照组给予注射同等剂量的生理盐水。模型制备成功后以振腹仪模拟振腹法进行治疗,之后观察大鼠的扭体反应及子宫体表投影温度,应用酶联免疫吸附试验测定血清中前列腺素F_(2α)、前列腺素E_(2)的含量。结果模型组扭体潜伏期短于对照组、扭体次数多于对照组及扭体评分高于对照组,差异有高度统计学意义(P<0.01);振腹组扭体潜伏期长于模型组、扭体次数少于模型组及扭体评分低于模型组,差异有统计学意义(P<0.05或P<0.01)。模型组子宫体表投影温度低于对照组,振腹组子宫体表投影温度高于模型组,差异有统计学意义(P<0.05或P<0.01)。模型组血清中前列腺素F_(2α)高于对照组,前列腺素E_(2)低于对照组,差异有统计学意义(P<0.05或P<0.01);振腹组血清中前列腺素F_(2α)低于模型组,前列腺素E_(2)高于模型组,差异有统计学意义(P<0.05或P<0.01)。结论振腹法可改善子宫局部温度失调状态,调控血清中前列腺素水平,抑制子宫平滑肌痉挛收缩,改善局部组织缺血缺氧进而达到“温经止痛”的作用。
Objective To investigate the effect of abdominal vibration on the projection temperature of uterus surface and prostaglandin E_(2) and prostaglandin F_(2α) of cold coagulation dysmenorrhea rats.Methods Thirty SD female six-week-old rats with body weight of(230±20)g were divided into control group,model group,and abdominal vibration group with ten rats in each group by random number table method.Model preparation was performed in model group and abdominal vibration group by freezing combined with subcutaneous injection of Estradiol Benzoate and intraperitoneal injection of Oxytocin combined with pain,while control group was injected with the same dose of normal saline.After the model was successfully prepared,abdominal vibration instrument was used to simulate the abdominal vibration method for treatment.After that,the writhing response of rats and the projection temperature of uterine surface were observed,and the levels of prostaglandin F_(2α) and prostaglandin E_(2) in serum were detected by enzyme linked immunosorbent assay.Results The writhing incubation period of the model group was shorter than that of the control group,the writhing number of the model group was more than that of the control group,and the writhing score of the model group was higher than that of the control group,and the differences were highly statistically significant(P<0.01).The writhing incubation period of the abdominal vibration group were longer than those of the model group,the writhing number of the abdominal vibration group was less than that in the model group,and the writhing score of the abdominal vibration group was lower than that of the model group,and the differences were statistically significant(P<0.05 or P<0.01).The uterine surface projection temperature in the model group was lower than that in the control group and the uterine surface projection temperature in the abdominal vibration group was higher than that in the model group,and the differences were statistically significant(P<0.05 or P<0.01).Serum prostaglandin F_(2α) in model group was higher than that in control group,and serum prostaglandin E_(2) was lower than that in control group,the differences were statistically significant(P<0.05 or P<0.01).The serum prostaglandin F_(2α) in abdominal vibration group was lower than that in model group,and the serum prostaglandin E_(2) was higher than that in model group,and the differences were statistically significant(P<0.05 or P<0.01).Conclusion Abdominal vibration can improve the local temperature imbalance of the uterus,regulate the level of prostaglandin in serum,inhibit the spasm and contraction of the smooth muscle of the uterus,improve local tissue ischemia and hypoxia,and thus achieve the effect of“warming womb and channels and relieving pain”.