文献类型：期刊文献

英文题名：Determination of Estrogen Residues in Milk Powder by Accelerated Solvent Extraction and Capillary Electrophoresis

作者：Gu, Chunxiu[1];Zhang, Wenjuan[1];Zhao, Youxi[1];Zhou, Kaowen[1]

第一作者：谷春秀

通讯作者：Zhao, YX[1];Zhou, KW[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2022

卷号：17

期号：11

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85140634486);WOS:【SCI-EXPANDED(收录号:WOS:000964494300011)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No.2152013) and Special Project of Education and Teaching Research and Reform of Beijing Union University (JJ2020Z012).

语种：英文

外文关键词：Estrogen; Milk powder; Accelerated solvent extraction; Capillary electrophoresis

摘要：A novel method which based on accelerated solvent extraction (ASE) and capillary electrophoresis (CE) was established for simultaneous determination of 7 estrogen residues in milk powders. During the ASE, the effects of extractant, extraction temperature and extraction time on extraction efficiency of 7 estrogens were studied. The optimized ASE process is as follows: mixing 1 g milk powder sample and 2 g diatomite in extraction tank, add 10 ml ethanol-acetonitrile (1:1, v/v), perform static extraction for 210 s at an extraction temperature of 120 degrees C, an extraction pressure of 10.0 MPa and cycle 3 times. During the CE separation, the effects of pH and ionic strength of the mobile phase, and separation voltage on the separation efficiency of 6 pair estrogens were investigated. The selected CE mobile phase is 50 mmol/L phosphate buffer solution (PBS) (pH=6.5) containing 25 mmol/ L NaCl, and separation voltage is 15 kV. All of 7 estrogens have good linear relationship. Their detection limits were 0.5 - 0.8 mu g/kg. Their recoveries in 3 milk powder samples were 84.7% - 114.8%.