文献类型：期刊文献

中文题名：番茄红素对高同型半胱氨酸血症大鼠血管内皮舒缩功能和纤溶功能的影响

英文题名：Effect of Lycopene on Vascular Endothelium and Fibrinolytic Function in Hyperhomocysteinemia Rats

作者：郭娴[1];李亮[2];曹建民[1];周海涛[3]

第一作者：郭娴

机构：[1]北京体育大学;[2]宿州学院;[3]北京联合大学生物化学工程学院

第一机构：北京体育大学,北京100084

年份：2015

卷号：27

期号：1

起止页码：41-44

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：安徽省教育厅自然科学重点研究项目(KJ2007A071)

语种：中文

中文关键词：番茄红素;大鼠;同型半胱氨酸;血管内皮;纤溶功能

外文关键词：lycopene; rats; homocysteine; vascular endothelium; fibrinolytic function

摘要：本文研究了补充番茄红素对高同型半胱氨酸血症大鼠血液Hcy的影响,及对血管内皮舒缩功能和纤溶功能的影响。SD大鼠30只,随机分为普通饮食组(C)、蛋氨酸饮食组(M)、蛋氨酸饮食+番茄红素组(ML)。ML组灌胃番茄红素(6.25 mg/kg),每天2 m L,其余组灌胃同等剂量生理盐水,共8周。末次实验后24 h,测试血浆Hcy、TXB2、6-Keto-PGF1α、t-PA和PAI。实验结果表明8周高蛋氨酸饮食可导致大鼠形成高同型半胱氨酸血症,血浆Hcy、TXB2和PAI显著升高(P<0.01),6-Keto-PGF1α和t-PA显著降低(P<0.01),血管舒缩和纤溶功能紊乱。但8周番茄红素补充可以显著降低血浆Hcy和TXB2,增加6-Keto-PGF1α含量,同时调节t-PA和PAI的活性,改善纤溶功能,预防动脉粥样硬化病的形成。
To observe the effect of lycopene on vascular endothelial and fibrinolytic function in hyperhomocysteinemia rats. Thirty male SD rats were randomly divided into the normal diet group（C）,methionine diet group（M）,methionine diet and lycopene group（ML）,with 10 rates in each group. The rats in C,M and ML groups were fed with normal diet,methionine diet,methionine diet,respectively. The methionine diet included 3% methionine. The rats in ML group were gavaged by lycopene with 6. 25 mg/kg ig volume of 2 m L. The rats in C and M groups were given saline of same volume.All the experiment lasted 8 weeks. 24 hours after the last experiment,the level of homocysteine,t-PA and PAI in blood or plasma were measured by radioimmunoassay. The level of TXB2and6-Keto-PGF1α in plasma were measured by enzyme linked immunosorbent assay（ELISA）. The results showed that after fed with 8-week methionine diet,rats had formed homocysteinemia. Compared with C group,plasma Hcy,TXB2 and PAI in M group were notably increased（P 0. 01）. At the mean time,6-Keto-PGF1α and t-PA were significantly decreased in M group（P 0. 01）. It indicated that the rats had happened vasomotor disorders and fibrinolytic activity disorders. After 8 weeks of lycopene supplement,compared with M group,plasma Hcy and TXB2 were significantly decreased in ML group（P 0. 01）,and 6-Keto-PGF1α were significantly increased in ML group（P 0. 01）. In addition,the rats in ML group had improved the activity of t-PA and PAI,the function of fibinolytic were enhanced to prevent the formation of atherosclerotic disease.